Construct: ORF TRCN0000475151

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001554.1_s317c1
Derived from:
ccsbBroadEn_11935
DNA Barcode:
TAAGCCTATCACCCTCATACCAAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ISOC1 (51015)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475151
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51015 ISOC1 isochorismatase domain cont... NM_016048.2 78.7% 78.8% 1_189del;298T>C
2 mouse 66307 Isoc1 isochorismatase domain cont... NM_025478.3 69.6% 77.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
771
ORF length:
705
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca ccgcaaattc gtggtgcagc tgttcgccga ggagtggggc cagtacgtgg
121acttgcccaa gggcttcgcg gtgagcgagc gctgcaaggt gcgcctcgtg ccgctgcaga
181tccagctcac taccctggga aatcttacac cttcaagcac tgtgtttttc tgctgtgata
241tgcaggaaag gttcagacca gccatcaagt attttgggga tattattagc gtgggacaga
301gattgttgca aggggcccgg attttaggaa ttcctgttat tgtaacagaa caatacccta
361aaggTCTTGG GAGCACGGTT CAAGAAATTG ATTTAACAGG TGTAAAACTG GTACTTCCAA
421AGACCAAGTT TTCAATGGTA TTACCAGAAG TAGAAGCGGC ATTAGCAGAG ATTCCCGGAG
481TCAGGAGTGT TGTATTATTT GGAGTAGAAA CTCATGTGTG CATCCAACAA ACTGCCCTGG
541AGCTAGTTGG CCGAGGAGTC GAGGTTCACA TTGTTGCTGA TGCCACCTCA TCAAGAAGCA
601TGATGGACAG GATGTTTGCC CTCGAGCGTC TCGCTCGAAC CGGGATCATA GTGACCACGA
661GTGAGGCTGT TCTGCTTCAG CTGGTAGCTG ATAAGGACCA TCCAAAATTC AAGGAAATTC
721AGAATCTAAT TAAGGCGAGT GCTCCAGAGT CGGGTCTGCT TTCCAAAGTA TACCCAACTT
781TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
841CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
901CTTGTGGAAA GGACGATAAG CCTATCACCC TCATACCAAG ACGCGTTAAG TCgacaatca
961acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)