Construct: ORF TRCN0000475170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001260.1_s317c1
- Derived from:
- ccsbBroadEn_10998
- DNA Barcode:
- AGCCCAACGAGTGCGATAGTGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NINJ2 (4815)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475170
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4815 | NINJ2 | ninjurin 2 | NM_016533.6 | 99.7% | 100% | 405C>A |
| 2 | human | 4815 | NINJ2 | ninjurin 2 | NM_001294345.2 | 93.2% | 92.2% | (many diffs) |
| 3 | human | 4815 | NINJ2 | ninjurin 2 | NM_001294346.2 | 74.4% | 74.6% | 0_1ins108;297C>A |
| 4 | human | 4815 | NINJ2 | ninjurin 2 | NM_001367996.1 | 74.4% | 74.6% | 0_1ins108;297C>A |
| 5 | human | 4815 | NINJ2 | ninjurin 2 | NR_160428.1 | 31.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 492
- ORF length:
- 426
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga atcagcaaga gaaaacatcg accttcaacc tggaagctcc gaccccagga 121 gccagcccat caaccTGAAC CATTACGCCA CCAAGAAGAG CGTGGCGGAG AGCATGCTGG 181 ACGTGGCCCT GTTCATGTCC AACGCCATGC GGCTGAAGGC GGTGCTGGAG CAGGGACCAT 241 CCTCTCACTA CTACACCACC CTGGTCACCC TCATCAGCCT CTCTCTGCTC CTGCAGGTGG 301 TCATCGGTGT CCTGCTCGTG GTCATTGCAC GGCTGAACCT GAATGAGGTA GAAAAGCAGT 361 GGCGACTCAA CCAGCTCAAC AACGCAGCCA CCATCTTGGT CTTCTTCACT GTGGTCATCA 421 ATGTTTTCAT TACAGCCTTC GGGGCACATA AAACAGGGTT CCTGGCTGCA AGGGCCTCAA 481 GGAATCCTCT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGC CCAACGAGTG CGATAGTGCG 661 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t