Construct: ORF TRCN0000475172
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017207.1_s317c1
- Derived from:
- ccsbBroadEn_14195
- DNA Barcode:
- ACAATCGTATCATGACCATCTACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PGM2 (55276)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475172
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55276 | PGM2 | phosphoglucomutase 2 | NM_018290.4 | 99.8% | .4% | 9delT;29G>A |
| 2 | human | 55276 | PGM2 | phosphoglucomutase 2 | XM_011513711.2 | 76.7% | .4% | (many diffs) |
| 3 | mouse | 66681 | Pgm1 | phosphoglucomutase 1 | NM_025700.2 | 84.2% | .3% | (many diffs) |
| 4 | mouse | 66681 | Pgm1 | phosphoglucomutase 1 | XM_006504057.3 | 78.2% | .3% | (many diffs) |
| 5 | mouse | 66681 | Pgm1 | phosphoglucomutase 1 | XM_006504058.2 | 63.8% | .4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 90
- ORF length:
- 24
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcccagaag gcagcggtct agacgaggac gcccggctgg accaggagac 121 cgcccagtgg ctgcgctggg acaagaattc cttaactttg gaggcagtga aacgactaat 181 agcagaaggt aataaagaag aactacgaaa atgttttggg gcccgaatgg agtttgggac 241 agctggcctc cgagctgcta tgggacctgg aatttctcgt atgaatgact tgaccatcat 301 ccagactaca cagggatttt gcagatacct ggaaaaacaa ttcagtgact taaagcagaa 361 aggcatcgtg atcagttttg acgcccgagc tcatccatcc agtgggggta gcagcagaag 421 gtttgcccga cttgctgcaa ccacatttat cagtcagggg attcctgtgt acctcttttc 481 tgatataacg ccaaccccct ttgtgccctt cacagtatca catttgaaac tttgtgctgg 541 aatcatgata actgcatctc acaatccaaa gcaggataat ggttataagg tctattggga 601 taatggagct cagatcattt ctcctcacga taaagggatt tctcaagcta ttgaagaaaa 661 tctagaaccg tggcctcaag cttgggacga ttctttaatt gatagcagtc cacttctcca 721 caatccgagt gcttccatca ataatgacta ctttgaagac cttaaaaagt actgtttcca 781 caggagcgtg aacagggaga caaaggtgaa gtttgtgcac acctctgtcc atggggtggg 841 tcatagcttt gtgcagtcag ctttcaaggc ttttgacctt gttcctcctg aggctgttcc 901 tgaacagaaa gatccggatc ctgagtttcc aacagtgaaa tacccgaatc ccgaagaggg 961 gaaaggtgtc ttgactttgt cttttgcttt ggctgacaaa accaaggcca gaattgtttt 1021 agctaacgac ccggatgctg atagacttgc tgtggcagaa aagcaagaca gtggtgaatg 1081 gagggtgttt tcaggcaatg agttgggggc cctcctgggc tggtggcttt ttacatcttg 1141 gaaagagaag aaccaggatc gcagtgctct caaagacacg tacatgttgt ccagcaccgt 1201 ctcctccaaa atcttgcggg ccattgcctt aaaggaaggt tttcattttg aggaaacatt 1261 aactggcttt aagtggatgg gaaacagagc caaacagcta atagaccagg ggaaaactgt 1321 tttatttgca tttgaagaag ctattggata catgtgctgc ccttttgttc tggacaaaga 1381 tggagtcagt gccgctgtca taagtgcaga gttGGCTAGC TTCCTAGCAA CCAAGAATTT 1441 GTCTTTGTCT CAGCAACTAA AGGCCATTTA TGTGGAGTAT GGCTACCATA TTACTAAAGC 1501 TTCCTATTTT ATCTGCCATG ATCAAGAAAC CATTAAGAAA TTATTTGAAA ACCTCAGAAA 1561 CTACGATGGA AAAAATAATT ATCCAAAAGC TTGTGGCAAA TTTGAAATTT CTGCCATTAG 1621 GGACCTTACA ACTGGCTATG ATGATAGCCA ACCTGATAAA AAAGCTGTTC TTCCCACTAG 1681 TAAAAGCAGC CAAATGATCA CCTTCACCTT TGCTAATGGA GGCGTGGCCA CCATGCGCAC 1741 CAGTGGGACA GAGCCCAAAA TCAAGTACTA TGCAGAGCTG TGTGCCCCAC CTGGGAACAG 1801 TGATCCTGAG CAGCTGAAGA AGGAACTGAA TGAACTGGTC AGTGCTATTG AAGAACATTT 1861 TTTCCAGCCA CAGAAGTACA ATCTGCAGCC AAAAGCAGAC TACCCAACTT TCTTGTACAA 1921 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 1981 ATGAACTAGT CCGTAACTTG AAAGTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 2041 CGAACAATCG TATCATGACC ATCTACCACG CGTTAAGTCg acaatcaacc tctggattac 2101 aaaatttgtg aaagatt