Construct: ORF TRCN0000475177
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007574.1_s317c1
- Derived from:
- ccsbBroadEn_05018
- DNA Barcode:
- GTCCGCCCCTCGCGGCGCAATAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM163A (148753)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475177
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148753 | FAM163A | family with sequence simila... | NM_001329712.2 | 100% | 100% | |
| 2 | human | 148753 | FAM163A | family with sequence simila... | NM_001329713.2 | 100% | 100% | |
| 3 | human | 148753 | FAM163A | family with sequence simila... | NM_001329714.2 | 100% | 100% | |
| 4 | human | 148753 | FAM163A | family with sequence simila... | NM_001329715.2 | 100% | 100% | |
| 5 | human | 148753 | FAM163A | family with sequence simila... | NM_001329716.2 | 100% | 100% | |
| 6 | human | 148753 | FAM163A | family with sequence simila... | NM_001329717.2 | 100% | 100% | |
| 7 | human | 148753 | FAM163A | family with sequence simila... | NM_001329718.2 | 100% | 100% | |
| 8 | human | 148753 | FAM163A | family with sequence simila... | NM_001329719.2 | 100% | 100% | |
| 9 | human | 148753 | FAM163A | family with sequence simila... | NM_173509.3 | 100% | 100% | |
| 10 | human | 148753 | FAM163A | family with sequence simila... | XM_006711174.2 | 100% | 100% | |
| 11 | human | 148753 | FAM163A | family with sequence simila... | XM_006711175.2 | 100% | 100% | |
| 12 | human | 148753 | FAM163A | family with sequence simila... | XM_006711177.1 | 100% | 100% | |
| 13 | human | 148753 | FAM163A | family with sequence simila... | XM_011509217.1 | 100% | 100% | |
| 14 | human | 148753 | FAM163A | family with sequence simila... | XM_017000386.1 | 100% | 100% | |
| 15 | human | 148753 | FAM163A | family with sequence simila... | XM_017000387.1 | 100% | 100% | |
| 16 | human | 148753 | FAM163A | family with sequence simila... | XM_017000388.1 | 100% | 100% | |
| 17 | human | 148753 | FAM163A | family with sequence simila... | XM_017000389.1 | 100% | 100% | |
| 18 | human | 148753 | FAM163A | family with sequence simila... | XM_017000390.1 | 100% | 100% | |
| 19 | human | 148753 | FAM163A | family with sequence simila... | XM_017000391.1 | 100% | 100% | |
| 20 | human | 148753 | FAM163A | family with sequence simila... | XM_017000392.1 | 100% | 100% | |
| 21 | human | 148753 | FAM163A | family with sequence simila... | XM_017000393.1 | 100% | 100% | |
| 22 | human | 148753 | FAM163A | family with sequence simila... | XM_024453399.1 | 100% | 100% | |
| 23 | mouse | 329274 | Fam163a | family with sequence simila... | NM_177838.3 | 87.6% | 84.6% | (many diffs) |
| 24 | mouse | 329274 | Fam163a | family with sequence simila... | XM_017321282.1 | 87.6% | 84.6% | (many diffs) |
| 25 | mouse | 329274 | Fam163a | family with sequence simila... | XM_017321283.1 | 87.6% | 84.6% | (many diffs) |
| 26 | mouse | 329274 | Fam163a | family with sequence simila... | XM_017321284.1 | 87.6% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 567
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agcgggaacg gttgtgatca ctggcggaat cctagctacg gtgatcctcc 121 tctgcatcat tgccgtcctg tgctactgca ggctccagta ttactgctgc aagaagagcg 181 gaaccgaggt tGCAGACGAG GAGGAGGAGC GGGAGCACGA CCTTCCCACG CATCCCAGAG 241 GCCCCACCTG CAATGCCTGC AGCTCCCAAG CCCTGGACGG CAGAGGCAGC CTGGCGCCTC 301 TCACCAGCGA GCCCTGCAGC CAGCCCTGTG GGGTGGCCGC GAGCCACTGC ACTACCTGCT 361 CCCCATACAG CTCCCCCTTT TACATACGGA CGGCTGACAT GGTGCCCAAT GGGGGTGGAG 421 GCGAGAGGCT CTCCTTTGCT CCCACATACT ACAAAGAGGG GGGACCCCCA TCCCTCAAAT 481 TGGCAGCACC CCAGAGTTAC CCGGTGACCT GGCCAGGCTC TGGGCGTGAG GCCTTCACCA 541 ATCCAAGGGC TATTAGTACA GACGTGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTCCGCCC 721 CTCGCGGCGC AATAAGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 781 aagatt