Construct: ORF TRCN0000475193
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017965.1_s317c1
- Derived from:
- ccsbBroadEn_09566
- DNA Barcode:
- TTACGGATGTACCCATGTAAGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SVOPL (136306)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475193
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 136306 | SVOPL | SVOP like | NM_174959.3 | 99.9% | 100% | 882A>G |
| 2 | human | 136306 | SVOPL | SVOP like | XM_011515797.2 | 99.9% | 100% | 882A>G |
| 3 | human | 136306 | SVOPL | SVOP like | XM_017011747.1 | 99.9% | 100% | 882A>G |
| 4 | human | 136306 | SVOPL | SVOP like | XM_017011748.1 | 99.9% | 100% | 882A>G |
| 5 | human | 136306 | SVOPL | SVOP like | NM_001331192.1 | 84.2% | 83.5% | (many diffs) |
| 6 | human | 136306 | SVOPL | SVOP like | XM_024446654.1 | 84.2% | 83.5% | (many diffs) |
| 7 | human | 136306 | SVOPL | SVOP like | XM_024446655.1 | 84.2% | 83.5% | (many diffs) |
| 8 | human | 136306 | SVOPL | SVOP like | NM_001139456.2 | 68.6% | 68.1% | (many diffs) |
| 9 | human | 136306 | SVOPL | SVOP like | XM_005250143.3 | 68.6% | 68.1% | (many diffs) |
| 10 | mouse | 320590 | Svopl | SV2 related protein homolog... | NM_177200.4 | 60.4% | 63% | (many diffs) |
| 11 | mouse | 320590 | Svopl | SV2 related protein homolog... | XM_011241386.2 | 54.5% | 56.6% | (many diffs) |
| 12 | mouse | 320590 | Svopl | SV2 related protein homolog... | XM_017321644.1 | 20.3% | 19.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgctggaagg ttaatcataa agactgaatt tttgcccacg aaataccgag 121 gctatatgtt acccttgtct caggtgttct ggcttgcggg ctccctgctc atcattggct 181 tggcctctgt gatcatcccc accatcgggt ggcgctggct cattcgcgtc gcctccatcc 241 cgggcatcat cctcatcgtg gccttcaagt ttattcctga atctgcccgg ttcaatgtct 301 ccactgggaa cactcgggct gccctggcca ctctggagcg cgttgccaag atgaaccgct 361 cggtcatgcc ggaggggaag ctggtggagc ccgtcctgga aaaaagagga agatttgcag 421 acctattgga tgctaaatat ttacggacca cattacagat ctgggtcata tggcttggaa 481 tctcttttgc ctactatggg gttatcctgg ccagtgctga gctgctggag cgggacttgg 541 tctgtggttc aaagtcagac tctgcggtgg tggtgactgg gggggactca ggggagagcc 601 agagcccctg ctactgccac atgtttgcac cctctgacta tcggaccatg atcatcagca 661 ccatcggtga aattgctttg aatcctttaa atatactggg catcaatttc ctgggaagac 721 ggctgagcct ttctattacc atgggatgca cggctttatt cttccttctc ctcaacattt 781 gcacttcaag tgccggccTG ATTGGCTTCC TCTTCATGCT GAGGGCTCTG GTAGCTGCAA 841 ACTTCAACAC CGTCTACATT TACACAGCTG AGGTCTACCC CACCACGATG CGCGCTTTGG 901 GGATGGGAAC CAGCGGCTCC CTGTGTCGCA TTGGTGCAAT GGTGGCGCCA TTTATATCCC 961 AGGTTCTTAT GAGTGCATCA ATACTGGGGG CCCTGTGTCT CTTCTCATCT GTCTGTGTTG 1021 TATGCGCCAT TTCTGCATTC ACTCTCCCCA TCGAAACCAA AGGACGGGCC CTCCAGCAAA 1081 TTAAATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTACGGATG TACCCATGTA AGGACACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt