Construct: ORF TRCN0000475198
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006156.1_s317c1
- Derived from:
- ccsbBroadEn_13482
- DNA Barcode:
- CATTAACTGAATTATCAAAAGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEGR1 (257194)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475198
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257194 | NEGR1 | neuronal growth regulator 1 | NM_173808.3 | 63.7% | 63.8% | 1_384del;888C>T |
| 2 | human | 257194 | NEGR1 | neuronal growth regulator 1 | XM_011541200.3 | 53.1% | 52.2% | (many diffs) |
| 3 | human | 257194 | NEGR1 | neuronal growth regulator 1 | XM_011541201.3 | 29% | 26.8% | (many diffs) |
| 4 | human | 257194 | NEGR1 | neuronal growth regulator 1 | XM_017000961.2 | 26.5% | 26.5% | 1_384del;666_667ins396 |
| 5 | mouse | 320840 | Negr1 | neuronal growth regulator 1 | NM_001039094.3 | 57.7% | 61.4% | (many diffs) |
| 6 | mouse | 320840 | Negr1 | neuronal growth regulator 1 | NM_177274.4 | 51.4% | 53% | (many diffs) |
| 7 | mouse | 320840 | Negr1 | neuronal growth regulator 1 | XM_006501577.1 | 50.5% | 51.9% | (many diffs) |
| 8 | mouse | 320840 | Negr1 | neuronal growth regulator 1 | XM_006501578.2 | 49.9% | 51.1% | (many diffs) |
| 9 | mouse | 320840 | Negr1 | neuronal growth regulator 1 | XM_006501576.2 | 46.7% | 48.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggtgcatcta actgtgcaag ttcctcctaa gatatatgac atctcaaatg 121 atatgaccgt caatgaagga accaacgtca ctcttacttg tttggccact gggaaaccag 181 agccttccat ttcttgGCGA CACATCTCCC CATCAGCAAA ACCATTTGAA AATGGACAAT 241 ATTTGGACAT TTATGGAATT ACAAGGGACC AGGCTGGGGA ATATGAATGC AGTGCGGAAA 301 ATGATGTGTC ATTCCCAGAT GTGAGGAAAG TAAAAGTTGT TGTCAACTTT GCTCCTACTA 361 TTCAGGAAAT TAAATCTGGC ACCGTGACCC CCGGACGCAG TGGCCTGATA AGATGTGAAG 421 GTGCAGGTGT GCCGCCTCCA GCCTTTGAAT GGTACAAAGG AGAGAAGAAG CTCTTCAATG 481 GCCAACAAGG AATTATTATT CAAAATTTTA GCACAAGATC CATTCTCACT GTTACCAACG 541 TGACACAGGA GCACTTCGGC AATTATACTT GTGTGGCTGC CAACAAGCTA GGCACAACCA 601 ATGCGAGCCT GCCTCTTAAC CCTCCAAGTA CAGCCCAGTA TGGAATTACC GGGAGCGCTG 661 ATGTTCTTTT CTCCTGCTGG TACCTTGTGT TGACACTGTC CTCTTTCACC AGCATATTCT 721 ACCTGAAGAA TGCCATTCTA CAATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 781 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 841 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ATTAACTGAA 901 TTATCAAAAG AACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 961 att