Construct: ORF TRCN0000475200
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014559.2_s317c1
- Derived from:
- ccsbBroadEn_15086
- DNA Barcode:
- GATGTGATACCGATATATACTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NMRK1 (54981)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475200
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | NM_017881.3 | 99.8% | 100% | 357T>G |
| 2 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_006717163.2 | 99.8% | 100% | 357T>G |
| 3 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_017014870.2 | 99.8% | 100% | 357T>G |
| 4 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | NM_001330678.2 | 95.7% | 92.3% | (many diffs) |
| 5 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | NM_001330679.2 | 95.7% | 92.3% | (many diffs) |
| 6 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_011518817.1 | 95.7% | 92.3% | (many diffs) |
| 7 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_017014868.1 | 95.7% | 92.3% | (many diffs) |
| 8 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_017014869.2 | 95.7% | 92.3% | (many diffs) |
| 9 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_024447590.1 | 95.7% | 92.3% | (many diffs) |
| 10 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | NM_001127603.2 | 87.9% | 87.4% | 317_318ins72 |
| 11 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_017014874.1 | 87.9% | 87.4% | 317_318ins72 |
| 12 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_017014871.1 | 84.1% | 80.2% | (many diffs) |
| 13 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_017014872.1 | 84.1% | 80.2% | (many diffs) |
| 14 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_017014873.1 | 84.1% | 80.2% | (many diffs) |
| 15 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_024447591.1 | 84.1% | 80.2% | (many diffs) |
| 16 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XM_024447592.1 | 84.1% | 80.2% | (many diffs) |
| 17 | human | 54981 | NMRK1 | nicotinamide riboside kinase 1 | XR_002956795.1 | 14% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa aacatttatc attggaatca gtggtgtgac aaacagtggc aaaacaacac 121 tggctaagaa tttgcagaaa cacctcccaa attgcagtgt catatctcag gatgatttct 181 tcaagccaga gtctgagata gagacagata aaaatggatt tttgcagtac gatgtgcttg 241 aagcacttaa catggaaaaa atgatgtcag ccatttccTG CTGGATGGAA AGCGCAAGAC 301 ACTCTGTGGT ATCAACAGAC CAGGAAAGTG CTGAGGAAAT TCCCATTTTA ATCATCGAAG 361 GTTTTCTTCT TTTTAATTAT AAGCCCCTTG ACACTATATG GAATAGAAGC TATTTCCTGA 421 CGATTCCATA TGAAGAATGT AAAAGGAGGA GGAGTACAAG GGTCTATCAG CCTCCAGACT 481 CTCCGGGATA CTTTGATGGC CATGTGTGGC CCATGTATCT AAAGTACAGA CAAGAAATGC 541 AGGACATCAC ATGGGAAGTT GTGTACCTGG ATGGAACAAA ATCTGAAGAG GACCTCTTTT 601 TGCAAGTATA TGAAGATCTA ATACAAGAAC TAGCAAAGCA AAAGTGTTTG CAAGTGACAG 661 CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGAGA TGTGATACCG ATATATACTC CCACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt