Construct: ORF TRCN0000475200

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014559.2_s317c1
Derived from:
ccsbBroadEn_15086
DNA Barcode:
GATGTGATACCGATATATACTCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NMRK1 (54981)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475200
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54981 NMRK1 nicotinamide riboside kinase 1 NM_017881.3 99.8% 100% 357T>G
2 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_006717163.2 99.8% 100% 357T>G
3 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_017014870.2 99.8% 100% 357T>G
4 human 54981 NMRK1 nicotinamide riboside kinase 1 NM_001330678.2 95.7% 92.3% (many diffs)
5 human 54981 NMRK1 nicotinamide riboside kinase 1 NM_001330679.2 95.7% 92.3% (many diffs)
6 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_011518817.1 95.7% 92.3% (many diffs)
7 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_017014868.1 95.7% 92.3% (many diffs)
8 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_017014869.2 95.7% 92.3% (many diffs)
9 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_024447590.1 95.7% 92.3% (many diffs)
10 human 54981 NMRK1 nicotinamide riboside kinase 1 NM_001127603.2 87.9% 87.4% 317_318ins72
11 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_017014874.1 87.9% 87.4% 317_318ins72
12 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_017014871.1 84.1% 80.2% (many diffs)
13 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_017014872.1 84.1% 80.2% (many diffs)
14 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_017014873.1 84.1% 80.2% (many diffs)
15 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_024447591.1 84.1% 80.2% (many diffs)
16 human 54981 NMRK1 nicotinamide riboside kinase 1 XM_024447592.1 84.1% 80.2% (many diffs)
17 human 54981 NMRK1 nicotinamide riboside kinase 1 XR_002956795.1 14% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
663
ORF length:
597
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa aacatttatc attggaatca gtggtgtgac aaacagtggc aaaacaacac
121tggctaagaa tttgcagaaa cacctcccaa attgcagtgt catatctcag gatgatttct
181tcaagccaga gtctgagata gagacagata aaaatggatt tttgcagtac gatgtgcttg
241aagcacttaa catggaaaaa atgatgtcag ccatttccTG CTGGATGGAA AGCGCAAGAC
301ACTCTGTGGT ATCAACAGAC CAGGAAAGTG CTGAGGAAAT TCCCATTTTA ATCATCGAAG
361GTTTTCTTCT TTTTAATTAT AAGCCCCTTG ACACTATATG GAATAGAAGC TATTTCCTGA
421CGATTCCATA TGAAGAATGT AAAAGGAGGA GGAGTACAAG GGTCTATCAG CCTCCAGACT
481CTCCGGGATA CTTTGATGGC CATGTGTGGC CCATGTATCT AAAGTACAGA CAAGAAATGC
541AGGACATCAC ATGGGAAGTT GTGTACCTGG ATGGAACAAA ATCTGAAGAG GACCTCTTTT
601TGCAAGTATA TGAAGATCTA ATACAAGAAC TAGCAAAGCA AAAGTGTTTG CAAGTGACAG
661CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
721TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
781GGCTTTATAT ATCTTGTGGA AAGGACGAGA TGTGATACCG ATATATACTC CCACGCGTTA
841AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)