Transcript: Human NM_001330678.2

Homo sapiens nicotinamide riboside kinase 1 (NMRK1), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
NMRK1 (54981)
Length:
2011
CDS:
429..1040

Additional Resources:

NCBI RefSeq record:
NM_001330678.2
NBCI Gene record:
NMRK1 (54981)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144748 CTATTCCATATAGTGTCAAG pXPR_003 GGG 336 55% 7 0.5363 NMRK1 NMRK1 75829
2 BRDN0001148142 AAACAGTGGCAAAACAACAC pXPR_003 TGG 64 10% 4 0.4124 NMRK1 NMRK1 75831
3 BRDN0001145652 ATATGACACTGCAATTTGGG pXPR_003 AGG 95 16% 4 -0.0002 NMRK1 NMRK1 75830
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001330678.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360130 CAGACTCTCCGGGATACTTTG pLKO_005 850 CDS 100% 10.800 15.120 N NMRK1 n/a
2 TRCN0000160469 CCCAACATGTGGAACAATAAA pLKO.1 1183 3UTR 100% 15.000 10.500 N NMRK1 n/a
3 TRCN0000360128 GGAAACTCCAAGGAGTAATTT pLKO_005 1077 3UTR 100% 15.000 10.500 N NMRK1 n/a
4 TRCN0000360131 ATATGGAATAGAAGCTATTTC pLKO_005 771 CDS 100% 13.200 9.240 N NMRK1 n/a
5 TRCN0000360129 GCAAGTATATGAAGATCTAAT pLKO_005 977 CDS 100% 13.200 9.240 N NMRK1 n/a
6 TRCN0000160781 CAAGACACTCTGTGGTATCAA pLKO.1 670 CDS 100% 5.625 3.938 N NMRK1 n/a
7 TRCN0000134781 CCTTCACCAAGATACAATGTA pLKO.1 1103 3UTR 100% 5.625 3.938 N NMRK1 n/a
8 TRCN0000160349 CTTGAAGCACTTAACATGGAA pLKO.1 612 CDS 100% 3.000 2.100 N NMRK1 n/a
9 TRCN0000134780 CTTCCTGAAGTGAATTAGGAA pLKO.1 1060 3UTR 100% 0.300 0.210 N NMRK1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001330678.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08439 pDONR223 100% 95.7% 92.3% None (many diffs) n/a
2 ccsbBroad304_08439 pLX_304 0% 95.7% 92.3% V5 (many diffs) n/a
3 TRCN0000479031 ACCAACATCTGTTACATTGGCACC pLX_317 63.3% 95.7% 92.3% V5 (many diffs) n/a
4 ccsbBroadEn_15086 pDONR223 0% 95.7% 92.3% None (many diffs) n/a
5 ccsbBroad304_15086 pLX_304 0% 95.7% 92.3% V5 (many diffs) n/a
6 TRCN0000475200 GATGTGATACCGATATATACTCCC pLX_317 64% 95.7% 92.3% V5 (many diffs) n/a
7 ccsbBroadEn_03503 pDONR223 100% 84.1% 80.2% None (many diffs) n/a
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