Construct: ORF TRCN0000475201
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018539.1_s317c1
- Derived from:
- ccsbBroadEn_02795
- DNA Barcode:
- CGGTCCGCGGCCATCACATTACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLDN14 (23562)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475201
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23562 | CLDN14 | claudin 14 | NM_001146077.1 | 100% | 100% | |
| 2 | human | 23562 | CLDN14 | claudin 14 | NM_001146078.3 | 100% | 100% | |
| 3 | human | 23562 | CLDN14 | claudin 14 | NM_001146079.2 | 100% | 100% | |
| 4 | human | 23562 | CLDN14 | claudin 14 | NM_012130.4 | 100% | 100% | |
| 5 | human | 23562 | CLDN14 | claudin 14 | NM_144492.3 | 100% | 100% | |
| 6 | mouse | 56173 | Cldn14 | claudin 14 | NM_001165925.1 | 86.5% | 93.3% | (many diffs) |
| 7 | mouse | 56173 | Cldn14 | claudin 14 | NM_001165926.1 | 86.5% | 93.3% | (many diffs) |
| 8 | mouse | 56173 | Cldn14 | claudin 14 | NM_019500.4 | 86.5% | 93.3% | (many diffs) |
| 9 | mouse | 56173 | Cldn14 | claudin 14 | XM_006523067.3 | 86.5% | 93.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcacggcc gtgcagcttc tgggcttcct gctcagcttc ctgggcatgg 121 tgggcacgtt gatcaccacc atcctgccgc actggcggag gacagcgcac gtgggcacca 181 acatcctcac ggccgtgtcc tacctgaaag ggctctggat ggagtgtgtg tggcacagca 241 caggcatcta ccagtgccag atctaccgat ccctgctggc gctgccccaa gacctccagg 301 ctgcccgcgc cctcatggtc atctcctgcc tgctctcggg catagcctgc gcctGCGCCG 361 TCATCGGGAT GAAGTGCACG CGCTGCGCCA AGGGCACACC CGCCAAGACC ACCTTTGCCA 421 TCCTCGGCGG CACCCTCTTC ATCCTGGCCG GCCTCCTGTG CATGGTGGCC GTCTCCTGGA 481 CCACCAACGA CGTGGTGCAG AACTTCTACA ACCCGCTGCT GCCCAGCGGC ATGAAGTTTG 541 AGATTGGCCA GGCCCTGTAC CTGGGCTTCA TCTCCTCGTC CCTCTCGCTC ATTGGTGGCA 601 CCCTGCTTTG CCTGTCCTGC CAGGACGAGG CACCCTACAG GCCCTACCAG GCCCCGCCCA 661 GGGCCACCAC GACCACTGCA AACACCGCAC CTGCCTACCA GCCACCAGCT GCCTACAAAG 721 ACAATCGGGC CCCCTCAGTG ACCTCGGCCA CGCACAGCGG GTACAGGCTG AACGACTACG 781 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGACG GTCCGCGGCC ATCACATTAC TAACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt