Construct: ORF TRCN0000475233

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008850.2_s317c1
Derived from:
ccsbBroadEn_14735
DNA Barcode:
GTCACCCACCACTCCTAAGATCAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDGFRL (5157)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475233
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5157 PDGFRL platelet derived growth fac... NM_006207.2 99.9% 100% 1041T>C
2 human 5157 PDGFRL platelet derived growth fac... NM_001372073.1 99.8% 100% 792T>C;1041T>C
3 mouse 68797 Pdgfrl platelet-derived growth fac... NM_026840.3 84.1% 89.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1191
ORF length:
1125
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa ggtctggctg ctgcttggtc ttctgctggt gcacgaagcg ctggaggatg
121ttactggcca acaccttccc aagaacaagc gtccaaaaga accaggagag aatagaatca
181aacctaccaa caagaaggtg aagcccaaaa ttcctaaaat gaaggacagg gactcagcca
241attcagcacc aaagacgcag tctatcatga tgcaagtgct ggataaaggt cgcttccaga
301aacccgccgc taccctgagt ctgctggcgg ggcaaactgt agagcttcga tgtaaaggga
361gtagaattgg gtggagctac cctgcgtatc tggacacctt taaggattct cgcctcagcg
421tcaagcagaa tgagcgctac ggccagttga ctctggtcaa ctccacctcg gcagacacag
481gtgaattcag ctgctgggtg cagctctgca gcggctacat ctgcaggaag gacgaggcca
541aaacgggctc cacctacatc ttttttacag agaaaggaga actctttgta ccttctccca
601gctacttcga tgttgtctac ttgaacccgg acagacaggc tgtggttcct tgtcgggtga
661ccgtgctgtc ggccaaagtc acgctccaca gggaattccc agccaaggag atcccagccA
721ATGGAACGGA CATTGTTTAT GACATGAAGC GGGGCTTTGT GTATCTGCAA CCTCATTCCG
781AGCACCAGGG TGTGGTTTAC TGCAGGGCGG AGGCCGGGGG CAGATCTCAG ATCTCCGTCA
841AGTACCAGCT GCTCTACGTG GCGGTTCCCA GTGGCCCTCC CTCAACAACC ATCTTGGCTT
901CTTCAAACAA AGTGAAAAGT GGGGACGACA TCAGTGTGCT CTGCACTGTC CTGGGGGAGC
961CCGATGTGGA GGTGGAGTTC ACCTGGATCT TCCCAGGGCA GAAGGATGAA AGGCCTGTGA
1021CGATCCAAGA CACTTGGAGG TTGATCCACA GAGGACTGGG ACACACCACG AGAATCTCCC
1081AGAGTGTCAT TACAGTGGAA GACTTCGAGA CGATTGATGC AGGATATTAC ATTTGCACTG
1141CTCAGAATCT TCAAGGACAG ACCACAGTAG CTACCACTGT TGAGTTTTCC TGCCCAACTT
1201TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1261CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1321CTTGTGGAAA GGACGAGTCA CCCACCACTC CTAAGATCAC ACGCGTTAAG TCgacaatca
1381acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)