Construct: ORF TRCN0000475240

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006294.1_s317c1
Derived from:
ccsbBroadEn_04104
DNA Barcode:
ACGACTTGCACTGCACGCAAACAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLEKHF2 (79666)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475240
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79666 PLEKHF2 pleckstrin homology and FYV... NM_024613.4 100% 100%
2 mouse 71801 Plekhf2 pleckstrin homology domain ... NM_175175.4 89.9% 97.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
813
ORF length:
747
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt ggatcgcttg gcaaacagtg aagcaaatac tagacgtata agtatagtgg
121aaaactgttt tggagcagct ggtcaacctt taactatacc tggacgagtt cttattggag
181aaggagtatt gactaagttg tgcaggaaaa agcccaaagc aaggcagttt ttcttgttta
241atgatattct tgtatatggc aatattgtca tccagaagaa aaaatataac aaacaacata
301ttattcccct ggaaaatgtc actattgatt ccatcaaaga tgagggagac ttaaggaatG
361GATGGCTAAT CAAGACACCA ACTAAATCTT TTGCAGTTTA TGCTGCCACT GCTACGGAGA
421AATCAGAATG GATGAATCAT ATAAATAAAT GTGTTACTGA TTTACTCTCC AAAAGTGGGA
481AGACACCCAG TAATGAACAT GCTGCTGTCT GGGTTCCTGA CTCTGAGGCA ACTGTATGTA
541TGCGTTGTCA GAAAGCAAAA TTCACACCTG TTAATCGTCG CCACCATTGC CGCAAATGTG
601GTTTTGTTGT CTGTGGGCCC TGCTCTGAAA AGAGATTTCT TCTTCCCAGC CAGTCCTCTA
661AGCCTGTGCG GATTTGTGAC TTCTGCTATG ACCTGCTTTC TGCTGGGGAC ATGGCCACAT
721GCCAGCCTGC TAGATCAGAC TCTTACAGCC AGTCATTGAA GTCTCCTTTA AATGATATGT
781CTGATGATGA TGACGATGAT GATAGCAGTG ACTACCCAAC TTTCTTGTAC AAAGTGGTTG
841ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
901GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC
961GACTTGCACT GCACGCAAAC ATACGCGTTA AGTCgacaat caacctctgg attacaaaat
1021ttgtgaaaga tt

Download FASTA (ORF) (Full)