Construct: ORF TRCN0000475240
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006294.1_s317c1
- Derived from:
- ccsbBroadEn_04104
- DNA Barcode:
- ACGACTTGCACTGCACGCAAACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHF2 (79666)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475240
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79666 | PLEKHF2 | pleckstrin homology and FYV... | NM_024613.4 | 100% | 100% | |
| 2 | mouse | 71801 | Plekhf2 | pleckstrin homology domain ... | NM_175175.4 | 89.9% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 813
- ORF length:
- 747
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggatcgcttg gcaaacagtg aagcaaatac tagacgtata agtatagtgg 121 aaaactgttt tggagcagct ggtcaacctt taactatacc tggacgagtt cttattggag 181 aaggagtatt gactaagttg tgcaggaaaa agcccaaagc aaggcagttt ttcttgttta 241 atgatattct tgtatatggc aatattgtca tccagaagaa aaaatataac aaacaacata 301 ttattcccct ggaaaatgtc actattgatt ccatcaaaga tgagggagac ttaaggaatG 361 GATGGCTAAT CAAGACACCA ACTAAATCTT TTGCAGTTTA TGCTGCCACT GCTACGGAGA 421 AATCAGAATG GATGAATCAT ATAAATAAAT GTGTTACTGA TTTACTCTCC AAAAGTGGGA 481 AGACACCCAG TAATGAACAT GCTGCTGTCT GGGTTCCTGA CTCTGAGGCA ACTGTATGTA 541 TGCGTTGTCA GAAAGCAAAA TTCACACCTG TTAATCGTCG CCACCATTGC CGCAAATGTG 601 GTTTTGTTGT CTGTGGGCCC TGCTCTGAAA AGAGATTTCT TCTTCCCAGC CAGTCCTCTA 661 AGCCTGTGCG GATTTGTGAC TTCTGCTATG ACCTGCTTTC TGCTGGGGAC ATGGCCACAT 721 GCCAGCCTGC TAGATCAGAC TCTTACAGCC AGTCATTGAA GTCTCCTTTA AATGATATGT 781 CTGATGATGA TGACGATGAT GATAGCAGTG ACTACCCAAC TTTCTTGTAC AAAGTGGTTG 841 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 901 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 961 GACTTGCACT GCACGCAAAC ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 1021 ttgtgaaaga tt