Construct: ORF TRCN0000475248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013218.1_s317c1
- Derived from:
- ccsbBroadEn_01654
- DNA Barcode:
- TGGCGGGTGCACAAAGTCTATTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCTA (6988)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475248
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6988 | TCTA | T cell leukemia translocati... | NM_022171.2 | 100% | 100% | |
| 2 | mouse | 102791 | Tcta | T cell leukemia translocati... | NM_001311156.1 | 86.4% | 89.6% | (many diffs) |
| 3 | mouse | 102791 | Tcta | T cell leukemia translocati... | NM_133986.2 | 75.1% | 77.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 399
- ORF length:
- 309
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGGAGTC CTGGTCTGGG CAGGCCTTGC 121 AGGCTCTGCC GGCCACGGTG CTGGGCGCGC TGGGCAGCGA GTTCTTGCGG GAGTGGGAGG 181 CGCAGGACAT GCGCGTGACC CTCTTCAAGC TGCTGCTGCT GTGGTTGGTG TTAAGTCTCC 241 TGGGCATCCA GCTGGCGTGG GGGTTCTACG GGAATACAGT GACCGGGTTG TATCACCGTC 301 CAGGTCTGGG TGGTCAGAAT GGATCCACGC CTGATGGCTC CACGCATTTC CCTTCGTGGG 361 AAATGGCAGC AAACGAACCT CTCAAAACCC ACAGAGAATA CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGATGGCGG GTGCACAAAG TCTATTCAAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt