Construct: ORF TRCN0000475261
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000692.1_s317c1
- Derived from:
- ccsbBroadEn_01973
- DNA Barcode:
- ATATCACTATGCGTGGTTATGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SKAP1 (8631)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475261
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8631 | SKAP1 | src kinase associated phosp... | NM_001075099.2 | 100% | 100% | |
| 2 | human | 8631 | SKAP1 | src kinase associated phosp... | NM_003726.4 | 99.7% | 99.7% | 876_878delAGT |
| 3 | human | 8631 | SKAP1 | src kinase associated phosp... | XM_005257755.4 | 95.2% | 95.2% | 824_825ins51 |
| 4 | human | 8631 | SKAP1 | src kinase associated phosp... | XM_024451012.1 | 94% | 84.1% | (many diffs) |
| 5 | human | 8631 | SKAP1 | src kinase associated phosp... | XM_017025257.2 | 93% | 81% | 0_1ins46;104_105ins26;804_806delAGT |
| 6 | human | 8631 | SKAP1 | src kinase associated phosp... | XM_017025258.1 | 74.6% | 74% | (many diffs) |
| 7 | human | 8631 | SKAP1 | src kinase associated phosp... | XM_017025259.1 | 73.5% | 73.5% | 0_1ins282;594_596delAGT |
| 8 | human | 8631 | SKAP1 | src kinase associated phosp... | XM_017025260.1 | 73.5% | 73.5% | 0_1ins282;594_596delAGT |
| 9 | mouse | 78473 | Skap1 | src family associated phosp... | NM_001033186.3 | 84.6% | 80.2% | (many diffs) |
| 10 | mouse | 78473 | Skap1 | src family associated phosp... | NM_001177899.1 | 81.6% | 77.9% | (many diffs) |
| 11 | mouse | 78473 | Skap1 | src family associated phosp... | XM_006534508.3 | 78.4% | 71.1% | (many diffs) |
| 12 | mouse | 78473 | Skap1 | src family associated phosp... | NM_001177898.1 | 75.5% | 68.9% | (many diffs) |
| 13 | mouse | 78473 | Skap1 | src family associated phosp... | XM_017314838.1 | 65.8% | 58.4% | (many diffs) |
| 14 | mouse | 78473 | Skap1 | src family associated phosp... | XM_017314839.1 | 62.9% | 56.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1140
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggccgccgcc ctccctgagg agatccgttg gctcctggaa gatgctgaag 121 agtttctggc agaaggtttg cggaatgaga acctcagcgc tgttgcaagg gatcacagag 181 accatattct acggggcttt cagcaaatca aagccaggta ctattgggat tttcagcccc 241 aagggggaga cattggacag gacagctctg atgataatca cagcgggact cttggcctgt 301 ccctcacatc cgatgcaccc tttttgtcag attatcagga tgagggaatg gaagacatcg 361 taaaaggagc tcaagaactt gataacgtaa tcaagcaagg atacttggag aagaaaagca 421 aagatcatag tttctttgga tcggagtggc agaagcgatg gtgtgttgtc agcagaggtc 481 tcttctacta ctatgctaat gagaagagca agcagcccaa agggaccttc ctcattaagg 541 gctacggtgt acggatggcc ccccacctgc gaagagattc caagaaagaa tcctgctttg 601 aactgacctc ccaggatagg cgcagctatg agtttacagc tactagTCCA GCAGAAGCCA 661 GAGACTGGGT GGATCAAATA AGTTTCTTGT TAAAGGATCT GAGCTCCTTA ACCATTCCAT 721 ATGAAGAGGA TGAGGAGGAA GAAGAAAAAG AAGAGACATA TGATGATATT GATGGTTTTG 781 ACTCCCCAAG TTGTGGTTCC CAGTGCAGAC CCACTATCTT GCCTGGGAGT GTGGGGATAA 841 AAGAGCCTAC AGAGGAGAAA GAAGAAGAAG ATATTTATGA AGTCTTGCCA GATGAAGAGC 901 ATGATCTAGA AGAGGATGAG AGTGGCACTC GACGAAAAGG AGACTATGCC AGTTACTACC 961 AGGGCCTATG GGATTGCCAT GGTGACCAGC CAGATGAACT GTCCTTCCAA CGGGGTGACC 1021 TCATCCGTAT TCTGAGCAAG GAGTATAACA TGTATGGCTG GTGGGTGGGA GAACTGAACA 1081 GCCTCGTTGG GATTGTTCCA AAGGAGTATC TCACCACTGC CTTTGAAGTG GAAGAAAGAT 1141 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGAATATC ACTATGCGTG GTTATGCAAA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt