Construct: ORF TRCN0000475274
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013948.1_s317c1
- Derived from:
- ccsbBroadEn_13193
- DNA Barcode:
- AGTATTCTCGATTGGGTAATCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM68 (137695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475274
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 137695 | TMEM68 | transmembrane protein 68 | NM_001286660.2 | 100% | 100% | |
| 2 | human | 137695 | TMEM68 | transmembrane protein 68 | NM_152417.3 | 50.3% | 45.5% | (many diffs) |
| 3 | human | 137695 | TMEM68 | transmembrane protein 68 | NM_001286657.2 | 40% | 36.1% | (many diffs) |
| 4 | human | 137695 | TMEM68 | transmembrane protein 68 | NM_001363176.1 | 40% | 36.1% | (many diffs) |
| 5 | human | 137695 | TMEM68 | transmembrane protein 68 | NR_156454.1 | 15.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat agacaaaaat caaacctgtg gtgtaggaca ggattctgtg ccctatatga 121 tttgtctgat tcacatactc gaagaatggt ttggtgtgga gcagttGGAG GACTATTTGA 181 ATTTTGCAAA CTATCTCTTG TGGGTTTTTA CACCACTAAT ACTTTTAATA CTTCCTTACT 241 TTACTATCTT TCTTCTCTAC CTTACTATTA TTTTCTTACA CATTTATAAG AGAAAGAATG 301 TATTGAAAGA AGCCTACTCT CATAATTTAT GGGATGGTGC AAGGAAAACA GTGGCAACTC 361 TGTGGGATGG ACATGCAGCC GTTTGGCATG GTAAGCAAGG ATACTTTCAC CTTTGTGTTG 421 CCATTCATGT GTGCTGCATT GGAACTGTGT TACCATTCCA TTTTATTGAT TGCCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATTC 601 TTGTGGAAAG GACGAAGTAT TCTCGATTGG GTAATCACAA CGCGTTAAGT Cgacaatcaa 661 cctctggatt acaaaatttg tgaaagatt