Construct: ORF TRCN0000475290
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007333.4_s317c1
- Derived from:
- ccsbBroadEn_01207
- DNA Barcode:
- TGACTGACATGCAAGACACCTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIP4K2A (5305)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475290
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5305 | PIP4K2A | phosphatidylinositol-5-phos... | NM_005028.5 | 100% | 100% | |
| 2 | human | 5305 | PIP4K2A | phosphatidylinositol-5-phos... | XM_006717450.2 | 87.4% | 87.4% | 335_336ins153 |
| 3 | human | 5305 | PIP4K2A | phosphatidylinositol-5-phos... | NM_001330062.1 | 85.4% | 85.4% | 0_1ins177 |
| 4 | human | 5305 | PIP4K2A | phosphatidylinositol-5-phos... | XM_017016330.1 | 85.4% | 85.4% | 0_1ins177 |
| 5 | human | 5305 | PIP4K2A | phosphatidylinositol-5-phos... | XM_017016331.1 | 85.4% | 85.4% | 0_1ins177 |
| 6 | human | 5305 | PIP4K2A | phosphatidylinositol-5-phos... | XM_017016332.1 | 72.9% | 72.9% | 0_1ins177;158_159ins153 |
| 7 | mouse | 18718 | Pip4k2a | phosphatidylinositol-5-phos... | NM_008845.4 | 89.2% | 98% | (many diffs) |
| 8 | mouse | 18718 | Pip4k2a | phosphatidylinositol-5-phos... | XM_006497394.3 | 78.3% | 86.2% | (many diffs) |
| 9 | mouse | 18718 | Pip4k2a | phosphatidylinositol-5-phos... | XM_006497395.3 | 78.2% | 85.7% | (many diffs) |
| 10 | mouse | 18718 | Pip4k2a | phosphatidylinositol-5-phos... | XM_006497396.3 | 75.2% | 83% | (many diffs) |
| 11 | mouse | 18718 | Pip4k2a | phosphatidylinositol-5-phos... | XM_006497397.3 | 54.7% | 60.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gacccccggc aacctagggt cctctgtcct ggcgagcaag accaagacca 121 agaagaagca cttcgtagcg cagaaagtga agctgtttcg ggccagcgac ccgctgctca 181 gcgtcctcat gtggggggta aaccactcga tcaatgaact gagccatgtt caaatccctg 241 ttatgttgat gccagatgac ttcaaagcct attcaaaaat aaaggtggac aatcaccttt 301 ttaacaaaga aaacatgccg agccatttca agtttaagga atactgcccg atggtcttcc 361 gtaacctgcg ggagaggttt ggaattgatg atcaagattt ccagaattcc ctgaccagga 421 gcgcacccct ccccaacgac tcccaggccc gcagtggagc tcgttttcac acttcctacg 481 acaaaagata catcatcaag actattacca gtgaagacgt ggccgaaatg cacaacatcc 541 tgaagaaata ccaccagtac atagtggaat gtcatgggat cacccttctt ccccagttct 601 tgggcatgta ccggcttaat gttgatggag ttgaaatata tgtgatagtt acaagaaatg 661 tattcagcca ccgtttgtct gtgtatagga aatacgactt aaagggctct acagtggcta 721 gagaagctag tgacaaagaa aaggccaaag aactgccaac tctgaaagat aatgatttca 781 ttaatgaggg ccaaaagatt tatattgatg acaacaacaa gaaggtcttc ctggaaaaac 841 taaaaaagga tgttgagttt ctggcccagc tgaagctcat ggacTACAGT CTGCTGGTGG 901 GAATTCATGA TGTGGAGAGA GCCGAACAGG AGGAAGTGGA GTGTGAGGAG AACGATGGGG 961 AGGAGGAGGG CGAGAGCGAT GGCACCCACC CGGTGGGAAC CCCCCCAGAT AGCCCCGGGA 1021 ATACACTGAA CAGCTCACCA CCCCTGGCTC CCGGGGAGTT CGATCCGAAC ATCGACGTCT 1081 ATGGAATTAA GTGCCATGAA AACTCGCCTA GGAAGGAGGT GTACTTCATG GCAATTATTG 1141 ACATCCTTAC TCATTATGAT GCAAAAAAGA AAGCTGCCCA TGCTGCAAAA ACTGTTAAAC 1201 ATGGCGCTGG CGCGGAGATC TCCACCGTGA ACCCAGAACA GTATTCAAAG CGCTTTTTGG 1261 ACTTTATTGG CCACATCTTG ACGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1381 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GACTGACATG 1441 CAAGACACCT TGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1501 att