Construct: ORF TRCN0000475290

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007333.4_s317c1
Derived from:
ccsbBroadEn_01207
DNA Barcode:
TGACTGACATGCAAGACACCTTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIP4K2A (5305)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475290
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5305 PIP4K2A phosphatidylinositol-5-phos... NM_005028.5 100% 100%
2 human 5305 PIP4K2A phosphatidylinositol-5-phos... XM_006717450.2 87.4% 87.4% 335_336ins153
3 human 5305 PIP4K2A phosphatidylinositol-5-phos... NM_001330062.1 85.4% 85.4% 0_1ins177
4 human 5305 PIP4K2A phosphatidylinositol-5-phos... XM_017016330.1 85.4% 85.4% 0_1ins177
5 human 5305 PIP4K2A phosphatidylinositol-5-phos... XM_017016331.1 85.4% 85.4% 0_1ins177
6 human 5305 PIP4K2A phosphatidylinositol-5-phos... XM_017016332.1 72.9% 72.9% 0_1ins177;158_159ins153
7 mouse 18718 Pip4k2a phosphatidylinositol-5-phos... NM_008845.4 89.2% 98% (many diffs)
8 mouse 18718 Pip4k2a phosphatidylinositol-5-phos... XM_006497394.3 78.3% 86.2% (many diffs)
9 mouse 18718 Pip4k2a phosphatidylinositol-5-phos... XM_006497395.3 78.2% 85.7% (many diffs)
10 mouse 18718 Pip4k2a phosphatidylinositol-5-phos... XM_006497396.3 75.2% 83% (many diffs)
11 mouse 18718 Pip4k2a phosphatidylinositol-5-phos... XM_006497397.3 54.7% 60.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1284
ORF length:
1218
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gacccccggc aacctagggt cctctgtcct ggcgagcaag accaagacca
121agaagaagca cttcgtagcg cagaaagtga agctgtttcg ggccagcgac ccgctgctca
181gcgtcctcat gtggggggta aaccactcga tcaatgaact gagccatgtt caaatccctg
241ttatgttgat gccagatgac ttcaaagcct attcaaaaat aaaggtggac aatcaccttt
301ttaacaaaga aaacatgccg agccatttca agtttaagga atactgcccg atggtcttcc
361gtaacctgcg ggagaggttt ggaattgatg atcaagattt ccagaattcc ctgaccagga
421gcgcacccct ccccaacgac tcccaggccc gcagtggagc tcgttttcac acttcctacg
481acaaaagata catcatcaag actattacca gtgaagacgt ggccgaaatg cacaacatcc
541tgaagaaata ccaccagtac atagtggaat gtcatgggat cacccttctt ccccagttct
601tgggcatgta ccggcttaat gttgatggag ttgaaatata tgtgatagtt acaagaaatg
661tattcagcca ccgtttgtct gtgtatagga aatacgactt aaagggctct acagtggcta
721gagaagctag tgacaaagaa aaggccaaag aactgccaac tctgaaagat aatgatttca
781ttaatgaggg ccaaaagatt tatattgatg acaacaacaa gaaggtcttc ctggaaaaac
841taaaaaagga tgttgagttt ctggcccagc tgaagctcat ggacTACAGT CTGCTGGTGG
901GAATTCATGA TGTGGAGAGA GCCGAACAGG AGGAAGTGGA GTGTGAGGAG AACGATGGGG
961AGGAGGAGGG CGAGAGCGAT GGCACCCACC CGGTGGGAAC CCCCCCAGAT AGCCCCGGGA
1021ATACACTGAA CAGCTCACCA CCCCTGGCTC CCGGGGAGTT CGATCCGAAC ATCGACGTCT
1081ATGGAATTAA GTGCCATGAA AACTCGCCTA GGAAGGAGGT GTACTTCATG GCAATTATTG
1141ACATCCTTAC TCATTATGAT GCAAAAAAGA AAGCTGCCCA TGCTGCAAAA ACTGTTAAAC
1201ATGGCGCTGG CGCGGAGATC TCCACCGTGA ACCCAGAACA GTATTCAAAG CGCTTTTTGG
1261ACTTTATTGG CCACATCTTG ACGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1321AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1381TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GACTGACATG
1441CAAGACACCT TGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1501att

Download FASTA (ORF) (Full)

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