Transcript: Mouse NM_008845.4

Mus musculus phosphatidylinositol-5-phosphate 4-kinase, type II, alpha (Pip4k2a), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Pip4k2a (18718)
Length:
3471
CDS:
127..1344

Additional Resources:

NCBI RefSeq record:
NM_008845.4
NBCI Gene record:
Pip4k2a (18718)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008845.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000415033 TGGGAATGTACCGGCTTAATG pLKO_005 662 CDS 100% 13.200 18.480 N Pip4k2a n/a
2 TRCN0000416303 GACTTGAAAGATATGAGATTG pLKO_005 1780 3UTR 100% 10.800 8.640 N Pip4k2a n/a
3 TRCN0000025578 CCATGAGAATGCGCCTAGAAA pLKO.1 1155 CDS 100% 5.625 4.500 N Pip4k2a n/a
4 TRCN0000025575 CCTTACTCATTACGATGCAAA pLKO.1 1206 CDS 100% 4.950 3.960 N Pip4k2a n/a
5 TRCN0000425695 GTATACTTCATGGCGATTATT pLKO_005 1180 CDS 100% 15.000 10.500 N Pip4k2a n/a
6 TRCN0000194659 CCGAGCCATTTCAAGTTTAAG pLKO.1 379 CDS 100% 13.200 9.240 N PIP4K2A n/a
7 TRCN0000025576 GCCGAGCCATTTCAAGTTTAA pLKO.1 378 CDS 100% 13.200 9.240 N Pip4k2a n/a
8 TRCN0000432717 TATTTCCCAGCATCGCTATTT pLKO_005 1521 3UTR 100% 13.200 9.240 N Pip4k2a n/a
9 TRCN0000025577 GCTGCCAACTTTAAAGGATAA pLKO.1 813 CDS 100% 10.800 7.560 N Pip4k2a n/a
10 TRCN0000413070 GCTTGAATCAGAGCTTGTAAC pLKO_005 1633 3UTR 100% 10.800 7.560 N Pip4k2a n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008845.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01207 pDONR223 100% 89.2% 98% None (many diffs) n/a
2 ccsbBroad304_01207 pLX_304 0% 89.2% 98% V5 (many diffs) n/a
3 TRCN0000475290 TGACTGACATGCAAGACACCTTGC pLX_317 33.1% 89.2% 98% V5 (many diffs) n/a
4 ccsbBroadEn_14766 pDONR223 0% 89.2% 98% None (many diffs) n/a
5 ccsbBroad304_14766 pLX_304 0% 89.2% 98% V5 (many diffs) n/a
6 TRCN0000471649 CGCTGGAGCCACCTTACCAGTTAT pLX_317 37.3% 89.2% 98% V5 (many diffs) n/a
Download CSV