Construct: ORF TRCN0000475291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003331.1_s317c1
- Derived from:
- ccsbBroadEn_08359
- DNA Barcode:
- CCAGAAATCCATACCTAACTATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRWD1 (54014)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475291
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | NM_001007246.2 | 99.7% | 99.1% | 247C>G |
| 2 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | XM_017028375.1 | 11.2% | 10.9% | (many diffs) |
| 3 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | XR_001754864.1 | 9.4% | (many diffs) | |
| 4 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | NM_033656.4 | 5.1% | 5% | (many diffs) |
| 5 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | XM_011529611.1 | 5.1% | 5% | (many diffs) |
| 6 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | NM_018963.5 | 5% | 4.9% | (many diffs) |
| 7 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | NM_176928.1 | 90.2% | 97.5% | (many diffs) |
| 8 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | NM_001103179.1 | 4.6% | 5% | (many diffs) |
| 9 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | NM_145125.3 | 4.5% | 4.9% | (many diffs) |
| 10 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | XM_011246157.2 | 4.5% | 4.9% | (many diffs) |
| 11 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | XM_017317169.1 | 3.9% | 4.2% | (many diffs) |
| 12 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | XM_017317170.1 | 3.9% | 4.2% | (many diffs) |
| 13 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | XM_006523114.3 | 3.8% | 4.1% | (many diffs) |
| 14 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | XM_006523113.1 | 3.8% | 4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 82
- ORF end:
- 442
- ORF length:
- 360
- Sequence:
-
1 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT 61 TTGTACAAAA AAGTTGGCAC CATGGCGGAG CCGTCGTCCG CCCGACGCCC GGTGCCTCTC 121 ATCGAGTCGG AGCTGTACTT CCTTATCGCC CGGTACCTAT CGGCGGGCCC GTGTCGGAGA 181 GCGGCCCAGG TGCTGGTGCA GGAGCTGGAG CAGTACCAGT TGTTGCCGAA GAGATTGGAC 241 TGGGAGGGCA ACGAGCACAA CAGGAGCTAC GAGGAGTTGG TCTTGTCCAA TAAGCATGTG 301 GCTCCTGATC ATCTTTTGCA AATCTGCGAG CGCATCGGTC CTATGTTGGA TAAAGAAATT 361 CCACCCAGTA TTTCAAGAGT CACTTCTTTA CTTGGTGCAG GAAGGCAGTC TTTGCTACGT 421 ACAGCAAAAG GTACCTTAAT TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 481 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 541 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCA GAAATCCATA 601 CCTAACTATT TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 661 t