Construct: ORF TRCN0000475294
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016215.1_s317c1
- Derived from:
- ccsbBroadEn_05779
- DNA Barcode:
- AGTGCCTGACGGAGGTTCGTGGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ADK (132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475294
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 132 | ADK | adenosine kinase | NM_001369124.1 | 54.9% | 39.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 aagccatgac gtcagtcaga gaaaatattc tctttggaat gggaaatcct ctgcttgaca 121 tctctgctgt agtggacaaa gatttccttg ataagtattc tctgaaacca aatgaccaaa 181 tcttggctga agacaaacac aaggaactgt ttgatgaact tgtgaaaaaa ttcaaagtcg 241 aatatcatgc tggtggctct acccagaatt caattaaagt ggctcagtgg atgattcaac 301 agccacacaa agcagcaaca ttttttggat gcattgggat agataaattt ggggagatcc 361 tgaagagaaa agctgctgaa gcccatgtgg atgctCCTTT CCTGAGAAGC CAGACTTCCA 421 CTGATGGAAG AGCTGAAAAC ACAAGCCCAG GAGTGCAGAC ACTGCCCTAA TTGCTTCCTG 481 AGAATTCCCA TATTAATAAA GAAGAAAATT ATCTGCCATT TTTTCCTACT ATAATAATGC 541 TGAATCTTAA TTTAGAGGGT ACAAGGGTAT GGTAATGCTT GTAGAATCTT TATTATCTCA 601 ACAATCTAAA AAATGATGTT TATTTCCATA GTTTGATAGT GCCACTTAAA TGCCAATTAA 661 ACAAGAATAT AACATTTCAA TAGAAAAAAA AAAAAAAAAA ACTCGAGGCA TCTATGTCGG 721 GTGCGGAGAA AGAGGTAATG AAATGGCACA TGGTCATAGC TGTTTCCTGA CCCAGCTTTC 781 TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT 841 ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 901 TGTGGAAAGG ACGAAGTGCC TGACGGAGGT TCGTGGCGAC GCGTTAAGTC gacaatcaac 961 ctctggatta caaaatttgt gaaagatt