Transcript: Human NM_001369124.1

Homo sapiens adenosine kinase (ADK), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
ADK (132)
Length:
1822
CDS:
111..977

Additional Resources:

NCBI RefSeq record:
NM_001369124.1
NBCI Gene record:
ADK (132)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147557 GTAGTAATGAGCATCCACAT pXPR_003 GGG 322 37% 5 1.2826 ADK ADK 75745
2 BRDN0001146463 TCTGGAGAAAAACTGGATGT pXPR_003 TGG 469 54% 6 0.5625 ADK ADK 75742
3 BRDN0001146410 ACAGCAGAGATGTCAAGCAG pXPR_003 AGG 48 6% 2 0.4083 ADK ADK 75743
4 BRDN0001146622 AAAGTCGAATATCATGCTGG pXPR_003 TGG 185 21% 4 0.1948 ADK ADK 75744
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001369124.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000382207 AGGGAGAGATGACACTATAAT pLKO_005 737 CDS 100% 15.000 10.500 N ADK n/a
2 TRCN0000379900 ATACACCTGATAGTCAAATAA pLKO_005 1454 3UTR 100% 15.000 10.500 N ADK n/a
3 TRCN0000315188 CTATGCAGCAAGCATCATAAT pLKO_005 911 CDS 100% 13.200 9.240 N ADK n/a
4 TRCN0000194650 CTCTTTAATGTGCACTCAAAC pLKO.1 1513 3UTR 100% 10.800 7.560 N ADK n/a
5 TRCN0000196377 GACAAAGATTTCCTTGATAAG pLKO.1 180 CDS 100% 10.800 7.560 N ADK n/a
6 TRCN0000024736 CCTTGATAAGTATTCTCTGAA pLKO.1 191 CDS 100% 4.950 3.465 N Adk n/a
7 TRCN0000280539 CCTTGATAAGTATTCTCTGAA pLKO_005 191 CDS 100% 4.950 3.465 N Adk n/a
8 TRCN0000010080 GACACTATAATGGCTACAGAA pLKO.1 747 CDS 100% 4.950 3.465 N ADK n/a
9 TRCN0000010076 ATATCATGCTGGTGGCTCTAC pLKO.1 287 CDS 100% 4.050 2.835 N ADK n/a
10 TRCN0000315254 ATATCATGCTGGTGGCTCTAC pLKO_005 287 CDS 100% 4.050 2.835 N ADK n/a
11 TRCN0000010079 GACATTAAAGAGATAGCCAAA pLKO.1 657 CDS 100% 4.050 2.835 N ADK n/a
12 TRCN0000010077 GAGCAGAATGAGCAGCCAACA pLKO.1 450 CDS 100% 4.050 2.835 N ADK n/a
13 TRCN0000315185 GAGCAGAATGAGCAGCCAACA pLKO_005 450 CDS 100% 4.050 2.835 N ADK n/a
14 TRCN0000315186 GCCACTTAAATGCCAATTAAA pLKO_005 1194 3UTR 100% 15.000 9.000 N ADK n/a
15 TRCN0000024734 GCTTTGAGACTAAAGACATTA pLKO.1 643 CDS 100% 13.200 7.920 N Adk n/a
16 TRCN0000297856 GCTTTGAGACTAAAGACATTA pLKO_005 643 CDS 100% 13.200 7.920 N Adk n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001369124.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05779 pDONR223 98.8% 83.4% 83.4% None 502_503ins171 n/a
2 ccsbBroad304_05779 pLX_304 0% 83.4% 83.4% V5 502_503ins171 n/a
3 TRCN0000475294 AGTGCCTGACGGAGGTTCGTGGCG pLX_317 53.1% 54.9% 39.2% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000488487 CCACACGCATTACAACTTATGGTG pLX_317 31% 83.4% 83.4% V5 (not translated due to prior stop codon) 502_503ins171 n/a
5 TRCN0000487814 ACCAATCACTTAAGGCCTCATGAG pLX_317 19.7% 83.3% 83.2% V5 156T>C;502_503ins171;864_865insG n/a
6 TRCN0000488966 CCTGAATGTTCTTAAATATCAGAC pLX_317 36.1% 79% 78.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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