Construct: ORF TRCN0000475304
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014368.1_s317c1
- Derived from:
- ccsbBroadEn_13789
- DNA Barcode:
- ACTTTCCTTACCCGCGCTCCAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475304
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55206 | SBNO1 | strawberry notch homolog 1 | NM_018183.5 | 12.3% | 10.7% | (many diffs) |
| 2 | human | 55206 | SBNO1 | strawberry notch homolog 1 | NM_001167856.3 | 12.3% | 10.7% | (many diffs) |
| 3 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530322.3 | 11.7% | 10.9% | (many diffs) |
| 4 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530323.3 | 11.7% | 10.9% | (many diffs) |
| 5 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530320.3 | 11.7% | 10.9% | (many diffs) |
| 6 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530321.3 | 11.7% | 10.9% | (many diffs) |
| 7 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530319.3 | 11.4% | 10.6% | (many diffs) |
| 8 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | NM_001081203.1 | 11.4% | 10.6% | (many diffs) |
| 9 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530317.3 | 11.4% | 10.6% | (many diffs) |
| 10 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530318.3 | 11.4% | 10.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggtgcatcag cagaatgcgt tatttcagta ttttgcggac acacttactg 121 cagttgttca aaatgccaaa aaaaatggaa gatatgatat gggaatctta gatcttggtt 181 ctGGAGATGA AAAAGTGCGG AAAAGTGATG TTAAAAAGTT TCTGACTCCA GGATATTCAA 241 CCTCTGGCCA CGTAGAATTA TACACAATTA GTGTAGAGAG GGGAATGTCA TGGGAGGAAG 301 CTACCAAGAT TTGGGCTGAG CTGACAGGAC CAGACGATGG CTTTTACTTG TCATTGCAAA 361 TAAGGAACAA CAAGAAAACT GCCATCTTAG TTAAAGAAGT GAATCCTAAA AAGAAACTTT 421 TCTTAGTTTA TCGACCAAAT ACTGGGAAGC AGCTCAAATT AGAAATTTAT GCTGATCTAA 481 AAAAGAAATA TAAGAAGGTA TTTTTTCATT TGTACATTAT ATTATATAGG AATACTTACT 541 TTGGGCCAGG CAATGTGGCT CACACCTGTA ATCTCAGCAC TTTGGGAGGC CGAGGTGGGC 601 AGATCACCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACTTTC CTTACCCGCG CTCCAGGCAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt