Construct: ORF TRCN0000475333
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004735.1_s317c1
- Derived from:
- ccsbBroadEn_13993
- DNA Barcode:
- CGGCCAAAGCTTCGAAATTAGGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SUPT3H (8464)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475333
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NM_181356.3 | 99.8% | 98.7% | 969delG |
| 2 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XM_011514949.3 | 97.1% | 96% | (many diffs) |
| 3 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XM_017011369.2 | 97.1% | 96% | (many diffs) |
| 4 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XM_017011370.1 | 97.1% | 96% | (many diffs) |
| 5 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NM_001350329.2 | 90.1% | 79.4% | (many diffs) |
| 6 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NM_003599.4 | 90.1% | 79.4% | (many diffs) |
| 7 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XM_017011371.1 | 90.1% | 79.4% | (many diffs) |
| 8 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XM_024446572.1 | 90.1% | 79.4% | (many diffs) |
| 9 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NM_001350326.2 | 90.1% | 89% | 35_36ins96;873delG |
| 10 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NM_001350325.2 | 89.5% | 78.9% | (many diffs) |
| 11 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NM_001350324.2 | 87.6% | 77% | (many diffs) |
| 12 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XM_011514951.3 | 87.6% | 77% | (many diffs) |
| 13 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XM_011514952.2 | 87.6% | 77% | (many diffs) |
| 14 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XM_011514953.3 | 87.6% | 77% | (many diffs) |
| 15 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XM_011514954.3 | 87.6% | 77% | (many diffs) |
| 16 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XR_926320.1 | 78.5% | 1_51del;885_1025del;1115_1116ins60 | |
| 17 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NM_001350327.2 | 70.3% | 69.2% | 0_1ins291;678delG |
| 18 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XR_926321.1 | 68.9% | (many diffs) | |
| 19 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XM_017011374.2 | 67.9% | 66.6% | (many diffs) |
| 20 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XR_926319.3 | 67.8% | 1_51del;1020delG;1036_1449del | |
| 21 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NR_146632.1 | 59.5% | (many diffs) | |
| 22 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NR_146634.1 | 56.2% | (many diffs) | |
| 23 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NR_146633.1 | 55.2% | (many diffs) | |
| 24 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NR_146635.1 | 54.6% | (many diffs) | |
| 25 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XR_001743692.1 | 53.4% | (many diffs) | |
| 26 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | NM_001261823.2 | 50.2% | 49% | 0_1ins489;480delG |
| 27 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XR_002956310.1 | 49.6% | (many diffs) | |
| 28 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XR_001743688.1 | 41.1% | (many diffs) | |
| 29 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XR_001743691.1 | 33.8% | (many diffs) | |
| 30 | human | 8464 | SUPT3H | SPT3 homolog, SAGA and STAG... | XR_001743685.1 | 32.8% | 1_51del;1020delG;1036_2992del | |
| 31 | mouse | 109115 | Supt3 | suppressor of Ty 3 | NM_178652.2 | 68.8% | 63.6% | (many diffs) |
| 32 | mouse | 109115 | Supt3 | suppressor of Ty 3 | XM_006523480.2 | 67.3% | 52.6% | (many diffs) |
| 33 | mouse | 109115 | Supt3 | suppressor of Ty 3 | XM_006523479.3 | 66% | 64.5% | (many diffs) |
| 34 | mouse | 109115 | Supt3 | suppressor of Ty 3 | XM_006523476.3 | 65.1% | 55.4% | (many diffs) |
| 35 | mouse | 109115 | Supt3 | suppressor of Ty 3 | XM_006523475.3 | 64.7% | 53.4% | (many diffs) |
| 36 | mouse | 109115 | Supt3 | suppressor of Ty 3 | XM_006523477.2 | 62.2% | 56.4% | (many diffs) |
| 37 | mouse | 109115 | Supt3 | suppressor of Ty 3 | XM_006523478.3 | 62.2% | 56.4% | (many diffs) |
| 38 | mouse | 109115 | Supt3 | suppressor of Ty 3 | XM_006523474.3 | 60.1% | 57.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 65
- ORF end:
- 1040
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgcatggtg gtctttggaa caacgttctt caactttgac agcttcatag ggaaaacaga 121 ttctatagaa ataattggaa aatcagtttt tccctattgt tatcacaata tccttccact 181 gctgaatgat tctggcaggt attctttagg tgatgctaga aggcctcttc atgaaacagc 241 agttttggta gaagatgtgg tacacactca gttaattaat ctgttacagc aagctgctga 301 agtttctcag ctgcggggag caagggtaat cactcctgaa gatcttctgt ttttgatgcg 361 caaagataag aaaaaactta gaagactgct aaaatacatg tttatccgag actacaaatc 421 aaagattgtc aaaggcatcg atgaggatga tcttctcgaa gacaaattga gtggcagcaa 481 taatgcgaAC AAAAGACAAA AGATTGCTCA GGACTTCCTC AACTCTATTG ACCAGACAGG 541 AGAACTTTTA GCAATGTTTG AAGATGACGA AATTGATGAA GTTAAACAAG AAAGAATGGA 601 GAGAGCAGAA AGACAAACTC GAATTATGGA TTCAGCTCAA TATGCAGAAT TCTGTGAAAG 661 TCGACAATTA AGTTTCTCCA AAAAAGCTTC CAAATTTCGA GACTGGTTGG ACTGCAGCAG 721 TATGGAGATA AAACCCAATG TTGTCGCAAT GGAAATCTTA GCATATTTAG CGTATGAAAC 781 TGTGGCACAG TTAGTGGATC TGGCTCTTCT TGTGAGGCAA GACATGGTAA CCAAGGCAGG 841 GGACCCCTTC AGCCATGCCA TTTCTGCAAC CTTCATTCAG TATCACAACT CTGCTGAGAG 901 CACTGCAGCC TGTGGTGTTG AGGCTCACAG CGATGCCATC CAGCCCTGCC ACATCAGAGA 961 GGCCATTCGA CGCTACAGCC ACAGGATTGG CCCACTTTCC CCATTCACAA ATGCCTACCG 1021 CAGGAATGGG ATGCTTTTCT AGCCTGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC 1081 GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG 1141 TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGGCCAA 1201 AGCTTCGAAA TTAGGCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg 1261 aaagatt