Construct: ORF TRCN0000475349
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013531.1_s317c1
- Derived from:
- ccsbBroadEn_05141
- DNA Barcode:
- CCCCTCTAGATCCTGCCCAAAGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UGT3A2 (167127)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475349
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 167127 | UGT3A2 | UDP glycosyltransferase fam... | NM_174914.4 | 100% | 100% | |
| 2 | human | 167127 | UGT3A2 | UDP glycosyltransferase fam... | XM_011513988.2 | 95% | 95% | 195_275del |
| 3 | human | 167127 | UGT3A2 | UDP glycosyltransferase fam... | NM_001168316.2 | 93.4% | 93.4% | 92_93ins102 |
| 4 | human | 167127 | UGT3A2 | UDP glycosyltransferase fam... | XM_017009150.1 | 92.7% | 92.7% | 0_1ins114 |
| 5 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | NM_152404.4 | 85.8% | 78.8% | (many diffs) |
| 6 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | XM_011513958.2 | 80.8% | 73.8% | (many diffs) |
| 7 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | XM_005248243.4 | 76.5% | 69.2% | (many diffs) |
| 8 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | XM_011513959.2 | 76.5% | 69.2% | (many diffs) |
| 9 | human | 167127 | UGT3A2 | UDP glycosyltransferase fam... | NR_031764.2 | 44.2% | 1_93del;404_405ins532;1131_1810del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1638
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgggcag cgagtgcttc ttctagtggg cttccttctc cctggggtcc 121 tgctctcaga ggctgccaaa atcctgacaa tatctacagt aggtggaagc cattatctac 181 tgatggaccg ggtttctcag attcttcaag atcacggtca taatgtcacc atgcttaacc 241 acaaaagagg tccttttatg ccagatttta aaaaggaaga aaaatcatat caagttatca 301 gttggcttgc acctgaagat catcaaagag aatttaaaaa gagttttgat ttctttctgg 361 aagaaacttt aggtggcaga ggaaaatttg aaaacttatt aaatgttcta gaatacttgg 421 cgttgcagtg cagtcatttt ttaaatagaa aggatatcat ggattcctta aagaatgaga 481 acttcgacat ggtgatagtt gaaacttttg actactgtcc tttcctgatt gctgagaagc 541 ttgggaagcc atttgtggcc attctttcca cttcattcgg ctctttggaa tttgggctac 601 caatcccctt gtcttatgtt ccagtattcc gttccttgct gactgatcac atggacttct 661 ggggccgagt gaagaatttt ctgatgttct ttagtttctg caggaggcaa cagcacatgc 721 agtctacatt tgacaacacc atcaaggaac atttcacaga aggctctagg ccagttttgt 781 ctcatcttct actgaaagca gagttgtggt tcattaactc tgactttgcc tttgattttg 841 ctcgacctct gcttcccaac actgtttatg ttggaggctt gatggaaaaa cctattaaac 901 cagtaccaca agacttggag aacttcattg ccaagtttgg ggactctggt tttgtccttg 961 tgaccttggg ctccatggtg aacacctgtc agaatccgga aatcttcaag gagatgaaca 1021 atgcctttgc tcacctaccc caaggggtga tatggaagtg tcagtgttct cattggccca 1081 aagatgtcca cctggctgca aatgtgaaaa ttgtggactg gcttcctcag agtgacctcc 1141 tggctcaccc aagcatccgt ctgtttgtca cccacggcgg gcagaatagc ataatggagg 1201 CCATCCAGCA TGGTGTGCCC ATGGTGGGGA TCCCTCTCTT TGGAGACCAG CCTGAAAACA 1261 TGGTCCGAGT AGAAGCCAAA AAGTTTGGTG TTTCTATTCA GTTAAAGAAG CTCAAGGCAG 1321 AGACATTGGC TCTTAAGATG AAACAAATCA TGGAAGACAA GAGATACAAG TCCGCGGCAG 1381 TGGCTGCCAG TGTCATCCTG CGCTCCCACC CGCTCAGCCC CACACAGCGG CTGGTGGGCT 1441 GGATTGACCA CGTCCTCCAG ACAGGGGGCG CGACGCACCT CAAGCCCTAT GTCTTTCAGC 1501 AGCCCTGGCA TGAGCAGTAC CTGCTCGACG TTTTTGTGTT TCTGCTGGGG CTCACTCTGG 1561 GGACTCTATG GCTTTGTGGG AAGCTGCTGG GCATGGCTGT CTGGTGGCTG CGTGGGGCCA 1621 GAAAGGTGAA GGAGACATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1681 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1741 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCCCTCT AGATCCTGCC 1801 CAAAGGGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt