Construct: ORF TRCN0000475362
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000343.1_s317c1
- Derived from:
- ccsbBroadEn_00789
- DNA Barcode:
- TGGCGCATCCTAAAGTGTAAGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSBP1 (3281)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475362
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3281 | HSBP1 | heat shock factor binding p... | NM_001537.4 | 100% | 100% | |
| 2 | mouse | 68196 | Hsbp1 | heat shock factor binding p... | NM_024219.1 | 85% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 237
- ORF end:
- 465
- ORF length:
- 228
- Sequence:
-
1 GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA 61 GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA 121 GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT 181 TCAGGTGTCG TGAGGCTAGC ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCATGG 241 CCGAGACTGA CCCCAAGACC GTGCAGGACC TCACCTCGGT GGTGCAGACA CTCCTGCAGC 301 AGATGCAAGA TAAATTTCAG ACCATGTCTG ACCAGATCAT TGGGAGAATT GATGATATGA 361 GTAGTCGCAT TGATGATCTG GAAAAGAATA TCGCGGACCT CATGACACAG GCTGGGGTGG 421 AAGAACTGGA AAGTGAAAAC AAGATACCTG CCACGCAAAA GAGTTGCCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA TGGCGCATCC TAAAGTGTAA GATTACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt