Construct: ORF TRCN0000475367
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009257.1_s317c1
- Derived from:
- ccsbBroadEn_08112
- DNA Barcode:
- AGGGCCACCATGGAACCACCTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM208 (29100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475367
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29100 | TMEM208 | transmembrane protein 208 | NM_014187.4 | 99.8% | 99.4% | 244G>T |
| 2 | human | 29100 | TMEM208 | transmembrane protein 208 | NR_134524.2 | 64.5% | (many diffs) | |
| 3 | human | 29100 | TMEM208 | transmembrane protein 208 | NM_001318217.2 | 59.3% | 58.9% | 0_1ins210;34G>T |
| 4 | mouse | 66320 | Tmem208 | transmembrane protein 208 | NM_025486.2 | 90.3% | 95.3% | (many diffs) |
| 5 | mouse | 66320 | Tmem208 | transmembrane protein 208 | XM_006531274.3 | 87.3% | 91.6% | (many diffs) |
| 6 | mouse | 66320 | Tmem208 | transmembrane protein 208 | XM_006531275.3 | 51.8% | 54.9% | (many diffs) |
| 7 | mouse | 66320 | Tmem208 | transmembrane protein 208 | XM_006531276.3 | 51.8% | 54.9% | (many diffs) |
| 8 | mouse | 66320 | Tmem208 | transmembrane protein 208 | XM_006531277.3 | 51.8% | 54.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 585
- ORF length:
- 519
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcccaagggc aaagtgggca cgagagggaa gaagcagata tttgaagaga 121 acagagagac tctgaagttc tacctgcgga tcatactggg ggccaatgcc atttactgcc 181 ttgtgacgtt ggtcttcttt tactcatctg cctcattttg ggcctggttg gccctgggct 241 ttagtctggc agtgtatggg gccagctacc actctatgag ctcgatggca cgagcagcgt 301 tctctgagta tggggccctg atggatggtg gcatggacct caacatggag cagggcatgg 361 cagagcacct taaggatgtg atcctactga cagccatcgt gcaggtgctc agctgcttct 421 ctctctatgt cTGGTCCTTC TGGCTTCTGG CTCCAGGCCG GGCCCTTTAC CTCCTGTGGG 481 TGAATGTGCT GGGCCCCTGG TTCACTGCAG ACAGTGGCAC CCCAGCACCA GAGCACAATG 541 AGAAACGGCA GCGCCGACAG GAGCGGCGGC AGATGAAGCG GTTATACCCA ACTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA AGGGCCACCA TGGAACCACC TTTAACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt