Construct: ORF TRCN0000475368
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007445.1_s317c1
- Derived from:
- ccsbBroadEn_12520
- DNA Barcode:
- CCCGTTGTTTGGAGAGTTATGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZBTB10 (65986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475368
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65986 | ZBTB10 | zinc finger and BTB domain ... | NM_001277145.2 | 80.8% | 79% | (many diffs) |
| 2 | human | 65986 | ZBTB10 | zinc finger and BTB domain ... | NM_001105539.3 | 80.1% | 80.1% | 1_519del |
| 3 | human | 65986 | ZBTB10 | zinc finger and BTB domain ... | NM_023929.4 | 77.3% | 77.3% | 1_519del;1995_1996ins72 |
| 4 | mouse | 229055 | Zbtb10 | zinc finger and BTB domain ... | NM_177660.3 | 71.6% | 71.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2163
- ORF length:
- 2094
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaggacggc gcgtccctga gcgacagcac cgaggacgag gaggaggggg 121 cgagcctggg cgacggcagc ggggcggaag gcggcagctg cagcagcagc aggcggtcgg 181 gcggcgatgg cggggacgaa gtggagggca gcggtgtggg agctggcgaa ggagagactg 241 tccagcactt cccgctcgcg cggcccaagt ctctaatgca gaagctccaa tgctccttcc 301 agacctcctg gctcaaggac tttccctggc tgcgctattc caaggatact ggtcttatgt 361 cttgcggctg gtgccaaaag acccctgcag atgggggaag cgtggacctt cccccagtgg 421 ggcatgatga gctttcgcga gggacccgca actacaagaa aaccctcctc ctgaggcacc 481 acgtctctac cgagcacaaa ctccacgaag ccaacgccca ggagtcagaa ataccatcag 541 aggaggggta ctgtgacttt aatagtaggc caaatgagaa ctcttattgc tatcaacttc 601 tgcgacaact aaatgaacag agaaagaaag gtattctttg tgatgtcagc attgtggtaa 661 gcggaaaaat cttcaaagct cataagaaca tcctggttgc aggcagccgt ttctttaaga 721 ctttatattg cttttcaaac aaagaaagcc ctaaccaaaa caatactacc cacttagata 781 ttgctgcagt tcaaggtttt tcagtcatct tggacttctt gtattctggt aacctggtgc 841 tcacaagcca aaatgccatt gaagttatga ccgtggccag ctatcttcaa atgagtgaag 901 ttgttcaaac ttgccgaaat ttcattaaag atgccttaaa tataagcatt aaatcagaag 961 ctccagagtc tgtagttgtg gactataata atagaaaacc agttaataga gatggtctgt 1021 cttcatcacg ggatcaaaaa attgccagtt tttgggcaac acggaatctt accaatttgg 1081 caagtaatgt aaagattgaa aatgatggtt gtaatgtcga cgagggccaa atagaaaact 1141 accaaatgaa tgacagtagt tgggtccagg atggatctcc tgaaatggct gaaaatgaat 1201 ctgaaggtca aacaaaagtg tttatttgga ataatatggg ctcccaggga attcaagaga 1261 ctggcaaaac aaggaggaaa aaccaaacta caaaaagatt tatttataat attccaccta 1321 ataatgaaac gaatttagaa gattgctcag taatgcagcc acctgttgcc tatccagaag 1381 aaaatacact actcatcaag gaagaaccag atttagatgg tgctctactc tcggggccag 1441 atggtgatag gaatgtgaat gcaaatttat tggctgaagc tggcactagt caagatggag 1501 gtgatgctgg tacttcacat gatttcaagt atggtttgat gcctggtcct tcaaatgatt 1561 tcaagtatgg attgatacca ggtacttcaa atgatttcaa gtatggattg ataccaggtg 1621 cttcaaatga tttcaagtat ggattattgc cagaatcttg gccaaaacaa gaaacctggg 1681 aaaatggtga atcatctcta atcatgaaca agttaaaatg ccctcattgt agctatgtag 1741 ccaaatacag acgaacacta aaaaggcact tgctcattca cacaggagtg agatcattta 1801 gctgtgatat ttgtggaaaa ctgtttactc gaagagaaca tgtaaaaaga cattccctgg 1861 tgcataaaaa ggataaaaaa tacaaatgta tggtgtgtaa gaagatcttc atgttagcag 1921 ccagtgttgg aataagacat ggatctcGAC GTTATGGTGT TTGTGTAGAC TGTGCAGATA 1981 AATCACAGCC AGGAGGGCAA GAAGGTGTAG ATCAGGGACA GGATACAGAA TTCCCTCGGG 2041 ATGAAGAATA CGAGGAGAAT GAAGTAGGAG AAGCTGATGA AGAGCTAGTT GATGATGGAG 2101 AAGATCAGAA TGATCCCTCT CGATGGGATG AATCAGGAGA AGTTTGTATG TCTCTAGATG 2161 ATTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2221 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2281 GGCTTTATAT ATCTTGTGGA AAGGACGACC CGTTGTTTGG AGAGTTATGA AGACGCGTTA 2341 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt