Construct: ORF TRCN0000475369
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002729.1_s317c1
- Derived from:
- ccsbBroadEn_04332
- DNA Barcode:
- CGGATCTAAAGCTCTTGGTTCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACAD11 (84129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475369
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84129 | ACAD11 | acyl-CoA dehydrogenase fami... | NM_032169.5 | 100% | 100% | |
| 2 | human | 84129 | ACAD11 | acyl-CoA dehydrogenase fami... | NR_132428.1 | 59.4% | 1_380del;1575_1576ins217;2504_3353del | |
| 3 | human | 84129 | ACAD11 | acyl-CoA dehydrogenase fami... | NR_132426.1 | 55.9% | 1_380del;2067_2676del;3331_4180del | |
| 4 | human | 84129 | ACAD11 | acyl-CoA dehydrogenase fami... | NR_132427.1 | 52.2% | (many diffs) | |
| 5 | human | 100532724 | NPHP3-ACAD11 | NPHP3-ACAD11 readthrough (N... | NR_037804.1 | 29.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2409
- ORF length:
- 2340
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagccaggt gctactggcg agtccgattt ggccgaagtg ctgccccagc 121 acaagttcga cagcaagtcc ctggaggcct acctaaacca gcacttgtct ggctttgggg 181 ccgaacgtga ggctacgctg accattgccc agtacagagc aggaaagtcc aatccaacct 241 tttatctcca gaagggcttt caaacatatg tgctcaggaa aaaaccacca ggttcacttc 301 ttcctaaagc acatcagatt gatagagaat ttaaagtcca gaaagccttg ttttcaattg 361 gattccccgt tcccaagcct atactgtact gcagtgatac ttctgtcatt ggaacagaat 421 tttacgtaat ggaacatgtg cagggtcgaa tcttccgtga tttaacaatt cctggactta 481 gcccagcaga acgttcagcc atatatgtgg ccacggtaga aacattggct cagttacatt 541 ccttgaatat acagtcactg cagctggaag gatatggtat aggtgctggg tactgcaaaa 601 gacaggtatc aacctggaca aagcaatatc aagctgcagc tcatcaggac atccctgcca 661 tgcaacagct atcggagtgg ctaatgaaga acttgcccga taatgacaat gaagagaatt 721 tgattcatgg agatttcaga ctagataaca tagttttcca ccctaaagag tgtcgagtta 781 tagcagtgct ggattgggag ctgtcaacca ttggtcatcc tttgtcagac ttagctcatt 841 tttccctgtt ctacttttgg ccaaggacag ttccaatgat aaatcaaggt tcttatagtg 901 aaaactcagg gataccatca atggaagaac tgatttcaat atattgccgc tgcaggggaa 961 ttaattctat tcttcctaac tggaatttct ttcttgccct ttcatatttt aagatggctg 1021 gaatagcaca gggagtatat agcagatatc ttctgggaaa taattcatct gaggatagct 1081 ttttatttgc caatattgtg caacctctgg cagaaactgg actacaactc tccaaacgaa 1141 ctttcagtac tgtactacca cagattgata ctactggaca gttgtttgta cagactcgga 1201 aaggtcagga agttcttatt aaggtgaagc atttcatgaa acaacacatt cttccagctg 1261 aaaaggaggt aactgagttc tatgttcaaa atgaaaattc agtggacaag tggggaaaac 1321 ctttagtgat tgataaactc aaggaaatgg ccaaagtcga gggtctctgg aacttgtttt 1381 tgccagctgt cagcggactc agccacgtgg actatgcctt gattgctgaa gaaacaggaa 1441 aatgcttttt tgctccagat gtctttaact gccaagcacc agacacaggg aatatggagg 1501 ttctgcacct gtatggaagt gaggaacaga agaaacagtg gcttgagcct cttcttcaag 1561 ggaacattac ctcttgcttc tgtatgacag aacctgatgt agcttcaagt gatgccacga 1621 atattgaatg cagcatccaa cgagatgaag atagctatgt aattaacggc aaaaaatggt 1681 ggagcagtgg agctgggaat cccaagtgca aaattgcaat tgttttggga agaactcaaa 1741 atacttctct ctccagacac aaacagcaca gcatgattct tgttcccatg aacacacctg 1801 gagtaaaaat aataaggcct ttgtcagttt ttggctacac agataatttt catggaggac 1861 attttgagat ccattttaat caagtgcgag ttcctgccac aaatctaata ctaggtgaag 1921 gtaggggatt tgaaatttcc caaggccgcc ttggaccTGG CAGAATCCAC CACTGTATGA 1981 GAACAGTAGG TTTGGCGGAA CGCGCTTTGC AGATCATGTG TGAGCGGGCA ACACAAAGGA 2041 TAGCTTTCAA GAAGAAGTTG TATGCACATG AGGTTGTGGC TCACTGGATT GCTGAAAGCC 2101 GCATTGCCAT TGAGAAGATC CGCTTGTTGA CTCTGAAAGC TGCTCACAGC ATGGACACTC 2161 TGGGCAGTGC TGGCGCTAAG AAAGAGATTG CAATGATCAA AGTGGCTGCC CCACGGGCTG 2221 TCAGCAAAAT CGTTGACTGG GCCATCCAGG TGTGCGGAGG TGCTGGTGTT TCCCAGGATT 2281 ACCCTCTGGC TAACATGTAT GCTATAACCC GAGTTTTGCG TTTAGCAGAT GGACCTGACG 2341 AAGTTCATCT TTCAGCAATC GCAACAATGG AGCTGCGGGA CCAAGCCAAA AGACTGACAG 2401 CCAAGATATT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2461 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2521 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGGATC TAAAGCTCTT GGTTCGTAAC 2581 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt