Construct: ORF TRCN0000475373
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001592.1_s317c1
- Derived from:
- ccsbBroadEn_11044
- DNA Barcode:
- CCGCCCCATAATATCGCCTCATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLR2A (5430)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475373
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5430 | POLR2A | RNA polymerase II subunit A | NM_000937.5 | 28.4% | 28.3% | (many diffs) |
| 2 | mouse | 20020 | Polr2a | polymerase (RNA) II (DNA di... | NM_001291068.1 | 25.9% | 28.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1764
- ORF length:
- 1698
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cgggggtggc cccccctcgg gggacagcgc atgcccgctg cgcaccatca 121 agagagtcca gttcggagtc ctgagtccgg atgaactgaa gcgaatgtct gtgacggagg 181 gtggcatcaa atacccagag acgactgagg gaggccgccc caagcttggg gggctgatgg 241 acccgaggca gggggtgatt gagcggactg gccgctgcca aacatgtgca ggaaacatga 301 cagagtgtcc tggccacttt ggccacattg aactggccaa gcctgtgttt cacgtgggct 361 tcctggtgaa gacaatgaaa gttttgcgct gtgtctgctt cttctgctcc aaactgcttg 421 tggactctaa caacccaaag atcaaggata tcctggctaa gtccaaggga cagcccaaga 481 agcggctcac acatgtctac gacctttgca agggcaaaaa catatgcgag ggtggggagg 541 agatggacaa caagttcggt gtggaacaac ctgagggtga cgaggatctg accaaagaaa 601 agggccatgg tggctgtggg cggtaccagc ccaggatccg gcgttctggc ctagagctgt 661 atgcggaatg gaagcacgtt aatgaggact ctcaggagaa gaagatcctg ctgagtccag 721 agcgagtgca tgagatcttc aaacgcatct cagatgagga gtgttttgtg ctgggcatgg 781 agccccgcta tgcacggcca gagtggatga ttgtcacagt gctgcctgtg cccccgctct 841 ccgtgcggcc tgctgttgtg atgcagggct ctgcccgtaa ccaggatgac ctgactcaca 901 aactggctga catcgtgaag atcaacaatc agctgcggcg caatgagcag aacggcgcag 961 cggcccatgt cattgcagag gatgtgaagc tcctccagtt ccatgtggcc accatggtgg 1021 acaacgagct gcctggcttg ccccgtgcca tgcagaagtc tgggcgtccc ctcaagtccc 1081 tgaagcagcg gttgaagggc aaggaaggcc gggtgcgagg gaacctgatg ggcaaaagag 1141 tggacttctc ggcccgtact gtcatcaccc ccgaccccaa cctctccatt gaccaggttg 1201 gcgtgccccg ctccattgct gccaacatga cctttgcgga gattgtcacc cccttcaaca 1261 ttgacagact tcaagaacta gtgcgcaggg ggaacagcca gtacccaggc gccaagtaca 1321 tcatccgaga caatggtgat cgcattgact tgcgtttcca ccccaagccc agtgaccttc 1381 acctgcagac cggctataag gtggaacggc acatgtgtga tggggacatt gttatcttca 1441 accggcagcc aactctgcac aaaatgtcca tgatggggca tcGGGTCCGC ATTCTCCCAT 1501 GGTCTACCTT TCGCTTGAAT CTTAGCGTGA CAACTCCGTA CAATGCAGAC TTTGACGGGG 1561 ATGAGATGAA CTTGCACCTG CCACAGTCTC TGGAGACGCG AGCAGAGATC CAGGAGCTGG 1621 CCATGGTTCC TCGCATGATT GTCACCCCCC AGAGCAATCG GCCTGTCATG GGTATTGTGC 1681 AGGACACACT CACAGCAGTG CGCAAATTCA CCAAGAGAGA CGTCTTCCTG GAGCGGGTGT 1741 GTGGTCCAAA TGGAAACCTG GCTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1801 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1861 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CGCCCCATAA 1921 TATCGCCTCA TTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1981 att