Construct: ORF TRCN0000475379

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017006.1_s317c1
Derived from:
ccsbBroadEn_11994
DNA Barcode:
CTCGCGACTGTCATGCACGGACCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GTSE1 (51512)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475379
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51512 GTSE1 G2 and S-phase expressed 1 NM_016426.7 97.3% 97.1% 1_57del;67C>T;1573T>C
2 human 51512 GTSE1 G2 and S-phase expressed 1 XM_005261627.4 97.3% 97.1% 1_57del;67C>T;1573T>C
3 human 51512 GTSE1 G2 and S-phase expressed 1 XM_017028816.2 75% 74.9% (many diffs)
4 human 51512 GTSE1 G2 and S-phase expressed 1 XM_011530213.3 64.1% 61.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2226
ORF length:
2160
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tgactctaag aaggaagaca ttcttctttt ggccgatgaa aaatttgact
121tcgatctttc attgtcttct tcgagtgcaa atgaagatga tgaagtcttc ttcggaccct
181ttggacataa agaaagatgt attgctgcca gcttggaatt aaataatccg gttcccgaac
241agcctccgtt gcccacatct gagagtccct ttgcctggag ccctctggcc ggggagaagt
301tcgtggaggt gtacaaagaa gctcacttac tggctttaca cattgagagc agcagccgga
361accaggcagc ccaagctgcc aagcctgaag accctcggag ccagggcgtg gaaagattca
421tacaggagtc aaaattaaaa ataaacctct ttgagaaaga aaaggaaatg aagaaaagcc
481ccacgtctct taaaagggag acatactacc tgtcagacag ccccttgctg gggccccctg
541tgggtgagcc tcggctcttg gcctcctccc cggccctgcc cagctctggt gcccaggccc
601gcctcacccg ggcgccgggg cctccgcact ctgctcatgc tttgcccagg gaatcatgca
661ctgctcatgc tgcaagtcag gcagcgactc agaggaagcc cgggaccaaa ttgctgctgc
721ctcgagcggc ctctgttaga ggaagaagca tccctggggc tgcggagaag cccaagaaag
781agattccagc tagtccttcc aggacaaaaa tcccagctga gaaggaatcc caccgggatg
841ttctccctga caaacctgcc ccgggtgctg tcaatgtgcc ggccgccgga agccacttgg
901gccagggcaa gcgggcgatc cctgttccaa acaagttggg gctgaagaag accctgttaa
961aagcacccgg ctctaccagc aatctcgcaa ggaagtcctc ctcggggcct gtttggagcg
1021gggcatccag tgcgtgcaca tccccagcag tgggcaaagc taaatcaagt gaatttgcaa
1081gtattcctgc aaatagctcc cggcctctgt caaacatcag caagtcaggc agaatgggac
1141ccgccatgct gcggccagct ctgcctgcag gccctgtggg ggcatcctcc tggcaggcca
1201agcgggtcga tgtttctgag ctggcagcgg agcagctcac ggcacccccc tcagcatccc
1261ccacccaacc ccagactccg gaaggtggcg gccagtggct gaactccagt tgcgcttggt
1321cagaatcttc tcaattgaat aagactagaa gtatcagacg gcgagattcc tgtctaaatt
1381ccaagacaaa ggttatgcct actcctacaa atcaatttaa aattcctaag ttttctattg
1441gtgactcccc ggacagctca acaccaaagc tttcgcgggc acagcggccg cagtcgtgca
1501cgtcagttgg cagggtcact gtccacagca ccccggttag acgctcatct gggccagcac
1561cacaaagcct gctgagcgca cggcgtgtgt cagccttgcc cacacccgcc agccggcgct
1621gctctggcct tccaccgatg acccccaaaa cgatgcccag ggccgtgggc tctcccctgt
1681gtgtgccagc tcggagacgt tcctctgagc cccgcaagaa ctctgCAATG AGAACTGAAC
1741CAACAAGGGA GAGCAACAGA AAGACAGATT CCAGGCTGGT GGATGTGTCC CCTGACAGGG
1801GTTCTCCTCC TTCCCGTGTG CCTCAGGCAC TTAACTTTTC TCCAGAGGAA AGCGATTCTA
1861CTTTCTCCAA AAGTACTGCC ACAGAAGTAG CTCGGGAGGA AGCCAAGCCG GGTGGAGATG
1921CAGCCCCTAG TGAGGCTCTT CTTGTAGATA TCAAACTGGA ACCACTCGCG GTCACTCCAG
1981ATGCTGCAAG CCAGCCCCTC ATTGACCTTC CTCTCATCGA CTTCTGCGAT ACCCCAGAAG
2041CACACGTGGC TGTAGGATCT GAAAGCAGGC CTCTGATCGA CCTCATGACA AACACTCCAG
2101ACATGAATAA AAATGTGGCC AAACCTTCAC CGGTGGTGGG ACAGCTCATA GACCTGAGCT
2161CCCCTCTGAT CCAGCTGAGC CCTGAGGCTG ACAAGGAGAA CGTGGATTCC CCACTCCTCA
2221AGTTCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
2281TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
2341CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTCGCGACT GTCATGCACG GACCCACGCG
2401TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)