Construct: ORF TRCN0000475386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003651.1_s317c1
- Derived from:
- ccsbBroadEn_10420
- DNA Barcode:
- CCTTCTGTACATCGGAATTTACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WASH8P (100288778)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475386
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_024447370.1 | 97.9% | 95.4% | (many diffs) |
| 2 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_017014171.1 | 67.8% | 61.4% | (many diffs) |
| 3 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_017014172.1 | 67.8% | 61.4% | (many diffs) |
| 4 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517666.2 | 58.7% | 57.2% | (many diffs) |
| 5 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517665.2 | 58.6% | 57.1% | (many diffs) |
| 6 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517664.3 | 57.3% | 55.8% | (many diffs) |
| 7 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_017014170.1 | 57.3% | 55.8% | (many diffs) |
| 8 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517663.3 | 57.2% | 55.7% | (many diffs) |
| 9 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_017014169.1 | 57.2% | 55.7% | (many diffs) |
| 10 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517662.3 | 55.7% | 54.3% | (many diffs) |
| 11 | human | 100287171 | WASHC1 | WASH complex subunit 1 | NM_182905.5 | 55.6% | 54.1% | (many diffs) |
| 12 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_024447369.1 | 55.6% | 54.1% | (many diffs) |
| 13 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517660.2 | 54.6% | 53.2% | (many diffs) |
| 14 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517659.2 | 54.5% | 53.1% | (many diffs) |
| 15 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517658.2 | 54% | 52.6% | (many diffs) |
| 16 | human | 102723897 | WASH9P | WAS protein family homolog ... | XR_001737556.1 | 44.1% | (many diffs) | |
| 17 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_002956737.1 | 43.6% | (many diffs) | |
| 18 | human | 653635 | WASH7P | WASP family homolog 7, pseu... | NR_024540.1 | 43.3% | (many diffs) | |
| 19 | human | 100288778 | WASH8P | WAS protein family homolog ... | NR_130745.1 | 41.7% | (many diffs) | |
| 20 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_002956741.1 | 41.1% | (many diffs) | |
| 21 | human | 375260 | WASH2P | WASP family homolog 2, pseu... | NR_024077.2 | 39.3% | (many diffs) | |
| 22 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_929142.2 | 36.2% | (many diffs) | |
| 23 | human | 374666 | WASH3P | WASP family homolog 3, pseu... | NR_003659.2 | 30.1% | (many diffs) | |
| 24 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_002956738.1 | 20.8% | (many diffs) | |
| 25 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_017014173.1 | 17.9% | 7.6% | (many diffs) |
| 26 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_002956739.1 | 10.2% | (many diffs) | |
| 27 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_002956740.1 | 10.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gggggcagag acagaggaga agctgtttga tgcccccttg tccatcagca 121 agagagagca gctggaacag caggtcccag agaactactt ctatgtgcca gacctgggcc 181 aggtgcctga gattgatgtt ccatcctacc tgcctgacct gcccagcatt gccaacgacc 241 tcatgtacat tgccgacctg ggccccggca ttgccccctc tgcccctggc accattccag 301 aactgcccac cttccacact gaggtagccg agcctctcaa ggcagaccta caagatgggg 361 tactaacacc acccccaccg cccccaccac cacccccagc tcctgaggtg ctggccagtg 421 cacccccact cccaccctca accgcggccc ctgtaggcca aggcgccagg caggacgaca 481 gcagcagcag cacgtctcct tcagtccagg gagctcccag ggaagtggtc gacccctcca 541 gtggctgggc cactctgcta gagtccatcc gccaagctgg gggcatcggc aaggccaagc 601 TGCGCAGCAT GAAGGAGCGA AAGCTGGAGA AGAAGAAGCA GAAGGAGCAG GAGCAAGTGA 661 GAGCCACGAG CCAAGGTGGG CACTTGATGT CGGATCTCTT CAACAAGCTG GTCATGAGGC 721 GCAAGGGCAT CTCTGGGAAA GGACCTGGGG CTGGTGAGGG GCCCGGAGGA GCCTTTGCCC 781 GCGTGTCAGA CTCCATCCCT CCTCTGCCGC CACCGCAGCA GCCACAGGTA GATGAGGACA 841 AGGACGACTG GGAATCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCTTCTG TACATCGGAA 1021 TTTACAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt