Construct: ORF TRCN0000475403
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010814.1_s317c1
- Derived from:
- ccsbBroadEn_11831
- DNA Barcode:
- TGATAGCGCTCTGCCTGGGTCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITGB1BP2 (26548)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475403
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26548 | ITGB1BP2 | integrin subunit beta 1 bin... | NM_012278.4 | 94.4% | 93.9% | (many diffs) |
| 2 | human | 26548 | ITGB1BP2 | integrin subunit beta 1 bin... | NM_001303277.3 | 68% | 68% | 0_1ins315 |
| 3 | mouse | 26549 | Itgb1bp2 | integrin beta 1 binding pro... | NM_013712.2 | 85.6% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1056
- ORF length:
- 987
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccacagat tcctgttgcc atcaccctgg ggtcccaatc ttccatgatg 121 cacttaaggg ttggtcctgc tgccgaaagc gaactgtaga tttctctgag ttcttaaaca 181 tcaagggctg tactatggga ccacactgtg ctgagaagct tcctgaggcc cctcaacctg 241 aaggccctgc tacaagcagt tcacttcagg agcaaaaacc tctgaatgtg attccaaagt 301 cagcagagac cttgcgccgg gagaggccca agtcagagtt gcctctgaag ctgctgccgc 361 taaatatatc ccaagccctg gaaatggcat tggaacagaa ggaattagac caggaacctg 421 gggcaggact tgacagtctg atccggactg gttccagctg ccagaaccca ggatgtgatg 481 ctgtttacca aggccctgag agtgatgcta ctccatgtac ctaccaccca ggagcacccc 541 gattccatga ggggatgaag tcttggagct gttgtggcat ccagaccctg gattttgggg 601 cattcttggc acaaccaggg tgcagagtcg gtagacatga ctgggggaag cagctcccag 661 catcttgccg ccatgattgg caccagacag attccttagt agtggtgact gtatatggcc 721 agattccact tcctgcgttt aactgggtga aggccagtca aactgagctt catgtccaca 781 ttgtctttga tggtaaccgt gtgttccaag cacagatgaa gctctggggg gtcataaacg 841 tggagcagag ctctgtcttc ttgatgccat ctcgggttga aatctccctg gtcAAGGCTG 901 ACCCAGGATC CTGGGCCCAG CTGGAGCACC CTGATGCACT AGCTAAGAAG GCTAGGGCAG 961 GGGTTGTGTT AGAGATGGAT GAGGAAGAAT CTGACGATTC AGATGATGAT CTGAGCTGGA 1021 CAGAGGAGGA GGAAGAGGAG GAAGCAATGG GGGAATTGCC AACTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 ATGATAGCGC TCTGCCTGGG TCAACACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt