Construct: ORF TRCN0000475407
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018332.1_s317c1
- Derived from:
- ccsbBroadEn_05827
- DNA Barcode:
- GAGGTACGTGATGTCCGAGGATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANXA13 (312)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475407
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 312 | ANXA13 | annexin A13 | NM_004306.4 | 99.7% | 99.3% | 257G>A;814G>A |
| 2 | human | 312 | ANXA13 | annexin A13 | NM_001003954.3 | 87.8% | 87.9% | (many diffs) |
| 3 | mouse | 69787 | Anxa13 | annexin A13 | NM_027211.2 | 85.2% | 86.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1017
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaatcgt catgctaaag cgagcagtcc tcagggtttt gatgtggatc 121 gagatgccaa aaagctgaac aaagcctgca aaggaatggg gaccaatgaa gcagccatca 181 ttgaaatctt atcgggcagg acatcagatg agaggcaaca aatcaagcaa aagtacaagg 241 caacgtacgg caaggagctg gaggaagtac tcaagagtga gctgagtgga aacttcgaga 301 agacagcgtt ggcccttctg gaccatccca gcgagtacgc cgcccggcag ctgcagaagg 361 ctatgaaggg tctgggcaca gatgagtccg tcctcattga ggtcctgtgc acgaggacca 421 ataaggaaat catcgccatt aaagaggcct accaaaggct atttgatagg agcctcgaat 481 cagatgtcaa aggtgataca agtggaaacc taaaaaaaat cctggtgtcT CTGCTGCAGG 541 CTAATCGCAA TGAAGGAGAT GACGTGGACA AAGATCTAGC TGGTCAGGAT GCCAAAGATC 601 TGTATGATGC AGGGGAAGGC CGCTGGGGCA CTGATGAGCT TGCGTTCAAT GAAGTCCTGG 661 CCAAGAGGAG CTACAAGCAG TTACGAGCCA CCTTTCAAGC CTATCAAATT CTCATTGGCA 721 AAGACATAGA AGAAGCCATT GAAGAAGAAA CATCAGGCGA CTTGCAGAAG GCCTATTTAA 781 CTCTCGTGAG ATGTGCCCAG GATTGTGAGG ACTATTTTGC TGAACGTCTG TACAAGTCGA 841 TGAAGGGTGC GGGGACCGAT GAGGAGACGT TGATTCGCAT AATCGTGACC AGGGCCGAGG 901 TGGACCTTCA GGGGATCAAA GCAAAGTTCC AAGAGAAGTA TCAGAAGTCT CTCTCTGACA 961 TGGTTCGCTC AGATACCTCC GGGGACTTCC GGAAACTGCT AGTAGCCCTC TTGCACTTGC 1021 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1081 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1141 ATATATCTTG TGGAAAGGAC GAGAGGTACG TGATGTCCGA GGATCCACGC GTTAAGTCga 1201 caatcaacct ctggattaca aaatttgtga aagatt