Construct: ORF TRCN0000475412
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007641.1_s317c1
- Derived from:
- ccsbBroadEn_00955
- DNA Barcode:
- GAAGCACCAGATGCTTATTTCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH2D1A (4068)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475412
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4068 | SH2D1A | SH2 domain containing 1A | NM_002351.4 | 100% | 100% | |
| 2 | human | 4068 | SH2D1A | SH2 domain containing 1A | NM_001114937.2 | 97.6% | 97.6% | 335_336insAGGTACTAC |
| 3 | mouse | 20400 | Sh2d1a | SH2 domain containing 1A | NM_011364.4 | 84.8% | 87.5% | (many diffs) |
| 4 | mouse | 20400 | Sh2d1a | SH2 domain containing 1A | NM_001313689.1 | 83.3% | 85.1% | (many diffs) |
| 5 | mouse | 20400 | Sh2d1a | SH2 domain containing 1A | NM_001313688.1 | 74.8% | 76% | (many diffs) |
| 6 | mouse | 20400 | Sh2d1a | SH2 domain containing 1A | NM_001313691.1 | 54.9% | 52.3% | (many diffs) |
| 7 | mouse | 20400 | Sh2d1a | SH2 domain containing 1A | XM_017318440.1 | 54.9% | 52.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgcagtggct gtgtatcatg gcaaaatcag cagggaaacc ggcgagaagc 121 tcctgcttgc cactgggctG GATGGCAGCT ATTTGCTGAG GGACAGCGAG AGCGTGCCAG 181 GCGTGTACTG CCTATGTGTG CTGTATCACG GTTACATTTA TACATACCGA GTGTCCCAGA 241 CAGAAACAGG TTCTTGGAGT GCTGAGACAG CACCTGGGGT ACATAAAAGA TATTTCCGGA 301 AAATAAAAAA TCTCATTTCA GCATTTCAGA AGCCAGATCA AGGCATTGTA ATACCTCTGC 361 AGTATCCAGT TGAGAAGAAG TCCTCAGCTA GAAGTACACA AGGTACTACA GGGATAAGAG 421 AAGATCCTGA TGTCTGCCTG AAAGCCCCAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAAGC 601 ACCAGATGCT TATTTCACTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt