Construct: ORF TRCN0000475425
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003635.1_s317c1
- Derived from:
- ccsbBroadEn_15953
- DNA Barcode:
- CGATCATCTGTGATCATAAGCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA4G (57715)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475425
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57715 | SEMA4G | semaphorin 4G | NM_001203244.1 | 100% | 100% | |
| 2 | human | 57715 | SEMA4G | semaphorin 4G | NM_017893.3 | 77.1% | 64.9% | (many diffs) |
| 3 | human | 57715 | SEMA4G | semaphorin 4G | XM_024448090.1 | 77.1% | 64.9% | (many diffs) |
| 4 | human | 57715 | SEMA4G | semaphorin 4G | XM_024448091.1 | 76.5% | 64.3% | (many diffs) |
| 5 | human | 57715 | SEMA4G | semaphorin 4G | XR_246101.3 | 48.4% | 1_458del;2162_3214del;3618_4345del | |
| 6 | human | 57715 | SEMA4G | semaphorin 4G | XR_945792.2 | 45.5% | (many diffs) | |
| 7 | mouse | 26456 | Sema4g | sema domain, immunoglobulin... | NM_011976.1 | 69.3% | 59% | (many diffs) |
| 8 | mouse | 26456 | Sema4g | sema domain, immunoglobulin... | XR_386479.1 | 40.9% | (many diffs) | |
| 9 | mouse | 26456 | Sema4g | sema domain, immunoglobulin... | XR_386477.1 | 38.8% | (many diffs) | |
| 10 | mouse | 26456 | Sema4g | sema domain, immunoglobulin... | XR_386476.2 | 38.7% | (many diffs) | |
| 11 | mouse | 26456 | Sema4g | sema domain, immunoglobulin... | XR_386478.1 | 37.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2175
- ORF length:
- 2106
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgggggagg ctctggcccc tcctcctcag catcctcaca gcaactgcag 121 tcccaggacc ctcactgcgg agaccgtcta gagaactaga tgccacccct cggatgacca 181 taccctatga agagctctct gggacccggc acttcaaggg ccaagcccag aactactcaa 241 cactgctgct ggaggaggcc tcagcaaggc tgctggtggg agcccgaggt gccctgttct 301 ctctcagtgc caacgacata ggagatgggg ctcacaaaga gatccactgg gaagcctccc 361 cagagatgca aagcaaatgt catcaaaaag ggaaaaacaa ccagacggag tgctttaacc 421 atgtgcggtt cctgcagcgg ctcaattcta cccacctcta tgcatgtggg actcacgcct 481 tccagcccct ctgtgcagcc attgatgctg aggccttcac cttgccaacc agcttcgagg 541 aggggaagga gaagtgtcct tatgacccag cccgtggctt cacaggcctc atcattgatg 601 gaggcctcta cacagccact aggtatgaat tccggagcat tcctgacatc cgccggagcc 661 gccacccaca ctccctgaga actgaggaga caccaatgca ttggctcaat gatgcggagt 721 ttgtgttctc cgtcctcgtg cgggagagca aggccagtgc agtgggtgat gatgacaagg 781 tgtactactt cttcacggag cgtgccactg aggagggctc tggcagcttc actcagagcc 841 gcagcagtca ccgtgtggcc cgtgtggctc gtgtctgcaa gggagacctg ggagggaaga 901 agatcctgca gaagaagtgg acttccttcc tgaaagcccg tctcatctgc cacattccac 961 tgtatgagac actgcgtggg gtctgcagcc tggatgctga aacctcaagc cgtacacact 1021 tctatgcagc cttcacgctg agcacacagt ggaagaccct ggaggcctca gccatctgcc 1081 gctatgacct ggcagagatc caggctgtct ttgcaggacc ctatatggaa taccaggatg 1141 gttcccggcg ctggggtcgc tatgagggtg gggtgcctga gccccggcct ggctcgtgta 1201 tcacagattc attgcgcagc caaggctaca attcatccca agacttgcca tccctggtcc 1261 tggactttgt aaagttgcac ccactgatgg ctcggcccgt tgtgcccaca cgtggacggc 1321 ccctgctgct caagcgcaac atacgctaca cacaccttac agggacacct gtcaccacgc 1381 ctgctggacc tacctatgac ctgctctttc tgggcacagc tgatggctgg atccacaagg 1441 ccgtagtcct gggctctggg atgcacatta ttgaagagac acaagtgttc agggagtccc 1501 agtctgtgga aaatctagtc atctctctat tgcagcacag cctctatgtg ggggctccta 1561 gcggagtcat ccagctacca ctctccagct gctcccgcta ccgatcctgc tatgactgca 1621 tcttggcccg agacccctac tgtggctggg accctggcac ccatgcctgc gcagcagcca 1681 ccaccatagc caacaggtcc cagggaagca ggacagcact gatacaggac atagagagag 1741 gaaatcgagg ctgtgagagc agcagggata caggcagggc tctgcaggtc catatgggct 1801 caatgtcacc accctctgca tggccctgtg tgctggatgg tcctgaaacc agacaagacc 1861 tctgccagcc acctaagccc tgcgtacatt cacatgcaca catggaagaa tgtttatcgg 1921 ctgggctgca gtgcccccac cctcaccttc tccTGGTGCA TTCTTGTTTC ATCCCTGCTT 1981 CTGGACTTGG GGTACCCTCC CAATTGCCAC ATCCTATCTG GTCCTCTTCC CCAGCCCCAT 2041 GTGGTGACCT CTTTGTCAAG AGCTTGGGAA CGGGCCAGCC TGGGGAGGTA AGACTGCATC 2101 ACTCCCCTCC TCTCCCTTCC TGTGTGGCCC TTGTGAATCA GCCTCCCCAC TCTCCTTGGT 2161 CATTCTCAAG AGTATTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2221 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2281 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGATCATCTG TGATCATAAG 2341 CCCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt