Construct: ORF TRCN0000475433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012670.1_s317c1
- Derived from:
- ccsbBroadEn_13841
- DNA Barcode:
- ACCCCTCCCGAATACTTATGAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP4A11 (1579)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475433
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284541 | CYP4A22 | cytochrome P450 family 4 su... | XM_005270770.3 | 76.2% | 72.2% | (many diffs) |
| 2 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | XM_005270539.1 | 60.1% | 57.3% | 606_865del;892C>T;906_1071del |
| 3 | human | 284541 | CYP4A22 | cytochrome P450 family 4 su... | NM_001308102.1 | 55.9% | 52.9% | (many diffs) |
| 4 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NM_001363587.2 | 50.9% | 48.6% | 606_865del;892C>T;906_1263del |
| 5 | human | 284541 | CYP4A22 | cytochrome P450 family 4 su... | XM_005270767.3 | 47.5% | 44.9% | (many diffs) |
| 6 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | XR_001737005.1 | 46.5% | (many diffs) | |
| 7 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NM_001319155.2 | 42.9% | 42.5% | (many diffs) |
| 8 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NM_000778.4 | 40.8% | 39.4% | (many diffs) |
| 9 | human | 284541 | CYP4A22 | cytochrome P450 family 4 su... | NM_001010969.4 | 39.3% | 36.4% | (many diffs) |
| 10 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134992.2 | 31.4% | (many diffs) | |
| 11 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134993.2 | 30.3% | (many diffs) | |
| 12 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134988.2 | 29.4% | (many diffs) | |
| 13 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134994.2 | 26.5% | (many diffs) | |
| 14 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134990.2 | 26.5% | (many diffs) | |
| 15 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134989.2 | 26.3% | (many diffs) | |
| 16 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | NR_134991.2 | 25.5% | (many diffs) | |
| 17 | human | 284541 | CYP4A22 | cytochrome P450 family 4 su... | XR_001737122.1 | 25.4% | (many diffs) | |
| 18 | human | 1579 | CYP4A11 | cytochrome P450 family 4 su... | XM_017000465.1 | 20.8% | 19.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 711
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgtctctgtg ctgagcccca gcagactcct gggtgatgtc tctggaatcc 121 tccaagcggc ctccctgctc attctgcttc tgctgctgat caaggcagtt cagctctacc 181 tgcacaggca gtggctgctc aaagccctcc agcagttccc gtgccctccc tcccactggc 241 tcttcgggca catccaggag ctccaacagg accaggagct acaacggatt cagaaatggg 301 tggagacatt cccaagtgcc tgtcctcatt ggctatgggg aggcaaagtT CGTGTCCAGC 361 TCTATGACCC TGACTATATG AAGGTGATTC TGGGGAGATC AGACCCGAAA TCCCATGGTT 421 CCTACAGATT CCTGGCTCCA TGGATTGGGT ACGGCTTGCT CCTGTTGAAT GGGCAGACAT 481 GGTTCCAGCA TCGACGGATG CTGACCCCAG CCTTCCACTA TGACATCCTG AAGCCCTATG 541 TGGGGCTCAT GGCAGACTCT GTACGAGTGA TGCTGGACAA ATGGGAAGAG CTCCTTGGCC 601 AGGATTCCCC TCTGGAGGTC TTTCAGCACG TCTCCTTGAT GACCCTGGAC ACCATCATGA 661 AGTGTGCCTT CAGGCATTGG CAGAGAGCTC AGCACTTCCG TCACCTTCCC TGCCCAACTT 721 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 781 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 841 CTTGTGGAAA GGACGAACCC CTCCCGAATA CTTATGAATA ACGCGTTAAG TCgacaatca 901 acctctggat tacaaaattt gtgaaagatt