Transcript: Human NM_000778.4

Homo sapiens cytochrome P450 family 4 subfamily A member 11 (CYP4A11), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
CYP4A11 (1579)
Length:
2530
CDS:
44..1603

Additional Resources:

NCBI RefSeq record:
NM_000778.4
NBCI Gene record:
CYP4A11 (1579)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000778.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000257423 TCCCGTGTGAGGAATGCCTTT pLKO_005 731 CDS 100% 4.050 5.670 N CYP4A11 n/a
2 TRCN0000244741 CAGAAATGGGTGGAGACATTC pLKO_005 269 CDS 100% 10.800 7.560 N CYP4A11 n/a
3 TRCN0000257406 CTGGGTGATGTCTCTGGAATC pLKO_005 77 CDS 100% 6.000 4.200 N CYP4A11 n/a
4 TRCN0000244740 CACCAGAATGACACCATCTAC pLKO_005 752 CDS 100% 4.950 3.465 N CYP4A11 n/a
5 TRCN0000244739 CTTGCTCTCCCAGGATCAATT pLKO_005 2063 3UTR 100% 13.200 6.600 Y CYP4A11 n/a
6 TRCN0000147049 CCACCTGTTTCTTTGTTTGAT pLKO.1 2203 3UTR 100% 5.625 2.813 Y CYP4A11 n/a
7 TRCN0000029419 CCTGACTATATGAAGGTGATT pLKO.1 347 CDS 100% 4.950 2.475 Y CYP4A22 n/a
8 TRCN0000029422 CGACTTGTGTTGAAATCCAAA pLKO.1 1520 CDS 100% 4.950 2.475 Y CYP4A22 n/a
9 TRCN0000029420 CGGGAAACAATTTGCCATGAA pLKO.1 1417 CDS 100% 4.950 2.475 Y CYP4A22 n/a
10 TRCN0000179008 CGGGAAACAATTTGCCATGAA pLKO.1 1417 CDS 100% 4.950 2.475 Y CYP4A11 n/a
11 TRCN0000029423 GCTGGAGAAGATCAAGAGGAA pLKO.1 880 CDS 100% 2.640 1.320 Y CYP4A22 n/a
12 TRCN0000282078 CAGCCTTCCACTATGACATTC pLKO_005 486 CDS 100% 10.800 6.480 N Cyp4a31 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000778.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13531 pDONR223 100% 85.3% 83.2% None (many diffs) n/a
2 ccsbBroad304_13531 pLX_304 0% 85.3% 83.2% V5 (many diffs) n/a
3 TRCN0000475714 GATATTATAATTATCCGTCACCAT pLX_317 22.5% 85.3% 83.2% V5 (many diffs) n/a
4 ccsbBroadEn_13841 pDONR223 100% 40.8% 39.4% None (many diffs) n/a
5 ccsbBroad304_13841 pLX_304 0% 40.8% 39.4% V5 (many diffs) n/a
6 TRCN0000475433 ACCCCTCCCGAATACTTATGAATA pLX_317 55.9% 40.8% 39.4% V5 (many diffs) n/a
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