Construct: ORF TRCN0000475435
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000186.3_s317c1
- Derived from:
- ccsbBroadEn_08116
- DNA Barcode:
- AGGCTGTCTGTAGTGTTCATCTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBK1 (29110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475435
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29110 | TBK1 | TANK binding kinase 1 | NM_013254.4 | 99.8% | 99.7% | 978T>A;1162A>G;1708A>C |
| 2 | human | 29110 | TBK1 | TANK binding kinase 1 | XM_005268809.1 | 99.8% | 99.7% | 978T>A;1162A>G;1708A>C |
| 3 | human | 29110 | TBK1 | TANK binding kinase 1 | XM_005268810.1 | 99.8% | 99.7% | 978T>A;1162A>G;1708A>C |
| 4 | human | 29110 | TBK1 | TANK binding kinase 1 | XR_001748674.2 | 69.4% | (many diffs) | |
| 5 | mouse | 56480 | Tbk1 | TANK-binding kinase 1 | NM_019786.4 | 86.2% | 93.8% | (many diffs) |
| 6 | mouse | 56480 | Tbk1 | TANK-binding kinase 1 | XR_001779563.1 | 50.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2253
- ORF length:
- 2187
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gagcacttct aatcatctgt ggcttttatc tgatatttta ggccaaggag 121 ctactgcaaa tgtctttcgt ggaagacata agaaaactgg tgatttattt gctatcaaag 181 tatttaataa cataagcttc cttcgtccag tggatgttca aatgagagaa tttgaagtgt 241 tgaaaaaact caatcacaaa aatattgtca aattatttgc tattgaagag gagacaacaa 301 caagacataa agtacttatt atggaatttt gtccatgtgg gagtttatac actgttttag 361 aagaaccttc taatgcctat ggactaccag aatctgaatt cttaattgtt ttgcgagatg 421 tggtgggtgg aatgaatcat ctacgagaga atggtatagt gcaccgtgat atcaagccag 481 gaaatatcat gcgtgttata ggggaagatg gacagtctgt gtacaaactc acagattttg 541 gtgcagctag agaattagaa gatgatgagc agtttgtttc tctgtatggc acagaagaat 601 atttgcaccc tgatatgtat gagagagcag tgctaagaaa agatcatcag aagaaatatg 661 gagcaacagt tgatctttgg agcattgggg taacatttta ccatgcagct actggatcac 721 tgccatttag accctttgaa gggcctcgta ggaataaaga agtgatgtat aaaataatta 781 caggaaagcc ttctggtgca atatctggag tacagaaagc agaaaatgga ccaattgact 841 ggagtggaga catgcctgtt tcttgcagtc tttctcgggg tcttcaggtt ctacttaccc 901 ctgttcttgc aaacatcctt gaagcagatc aggaaaagtg ttggggtttt gaccagtttt 961 ttgcagaaac tagtgatata cttcaccgaa tggtaattca tgttttttcg ctacaacaaa 1021 tgacagctca taagatttat atacatagct ataatactgc tactatattt catgaactgg 1081 tatataaaca aaccaaaatt atttcttcaa atcaagaact tatctacgaa gggcgacgct 1141 tagtcttaga acctggaagg ctggcacaac atttccctaa aactactgag gaaaacccta 1201 tatttgtagt aagccgggaa cctctggata ccataggatt aatatatgaa aaaatttccc 1261 tccctaaagt acatccacgt tatgatttag acggggatgc tagcatggct aaggcaataa 1321 caggggttgt gtgttatgcc tgcagaattg ccagtacctt actgctttat caggaattaa 1381 tgcgaaaggg gatacgatgg ctgattgaat taattaaaga tgattacaat gaaactgttc 1441 acaaaaagac agaagttgtg atcacattgg atttctgtat cagaaacatt gaaaaaactg 1501 tgaaagtata tgaaaagttg atgaagatca acctggaagc ggcagagtta ggtgaaattt 1561 cagacataca caccaaattg ttgagacttt ccagttctca gggaacaata gaaaccagtc 1621 ttcaggatat cgacagcaga ttatctccag gtggatcact ggcagacgca tgggcacatc 1681 aagaaggcac tcatccGAAA GACAGAAATG TAGAAAAACT ACAAGTCCTG TTAAATTGCA 1741 TGACAGAGAT TTACTATCAG TTCAAAAAAG ACCAAGCAGA ACGTAGATTA GCTTATAATG 1801 AAGAACAAAT CCACAAATTT GATAAGCAAA AACTGTATTA CCATGCCACA AAAGCTATGA 1861 CGCACTTTAC AGATGAATGT GTTAAAAAGT ATGAGGCATT TTTGAATAAG TCAGAAGAAT 1921 GGATAAGAAA GATGCTTCAT CTTAGGAAAC AGTTATTATC GCTGACTAAT CAGTGTTTTG 1981 ATATTGAAGA AGAAGTATCA AAATATCAAG AATATACTAA TGAGTTACAA GAAACTCTGC 2041 CTCAGAAAAT GTTTACAGCT TCCAGTGGAA TCAAACATAC CATGACCCCA ATTTATCCAA 2101 GTTCTAACAC ATTAGTAGAA ATGACTCTTG GTATGAAGAA ATTAAAGGAA GAGATGGAAG 2161 GGGTGGTTAA AGAACTTGCT GAAAATAACC ACATTTTAGA AAGGTTTGGC TCTTTAACCA 2221 TGGATGGTGG CCTTCGCAAC GTTGACTGTC TTTACCCAAC TTTCTTGTAC AAAGTGGTTG 2281 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2341 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 2401 GCTGTCTGTA GTGTTCATCT CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 2461 ttgtgaaaga tt