Construct: ORF TRCN0000475435

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000186.3_s317c1
Derived from:
ccsbBroadEn_08116
DNA Barcode:
AGGCTGTCTGTAGTGTTCATCTCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TBK1 (29110)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475435
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29110 TBK1 TANK binding kinase 1 NM_013254.4 99.8% 99.7% 978T>A;1162A>G;1708A>C
2 human 29110 TBK1 TANK binding kinase 1 XM_005268809.1 99.8% 99.7% 978T>A;1162A>G;1708A>C
3 human 29110 TBK1 TANK binding kinase 1 XM_005268810.1 99.8% 99.7% 978T>A;1162A>G;1708A>C
4 human 29110 TBK1 TANK binding kinase 1 XR_001748674.2 69.4% (many diffs)
5 mouse 56480 Tbk1 TANK-binding kinase 1 NM_019786.4 86.2% 93.8% (many diffs)
6 mouse 56480 Tbk1 TANK-binding kinase 1 XR_001779563.1 50.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2253
ORF length:
2187
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca gagcacttct aatcatctgt ggcttttatc tgatatttta ggccaaggag
121ctactgcaaa tgtctttcgt ggaagacata agaaaactgg tgatttattt gctatcaaag
181tatttaataa cataagcttc cttcgtccag tggatgttca aatgagagaa tttgaagtgt
241tgaaaaaact caatcacaaa aatattgtca aattatttgc tattgaagag gagacaacaa
301caagacataa agtacttatt atggaatttt gtccatgtgg gagtttatac actgttttag
361aagaaccttc taatgcctat ggactaccag aatctgaatt cttaattgtt ttgcgagatg
421tggtgggtgg aatgaatcat ctacgagaga atggtatagt gcaccgtgat atcaagccag
481gaaatatcat gcgtgttata ggggaagatg gacagtctgt gtacaaactc acagattttg
541gtgcagctag agaattagaa gatgatgagc agtttgtttc tctgtatggc acagaagaat
601atttgcaccc tgatatgtat gagagagcag tgctaagaaa agatcatcag aagaaatatg
661gagcaacagt tgatctttgg agcattgggg taacatttta ccatgcagct actggatcac
721tgccatttag accctttgaa gggcctcgta ggaataaaga agtgatgtat aaaataatta
781caggaaagcc ttctggtgca atatctggag tacagaaagc agaaaatgga ccaattgact
841ggagtggaga catgcctgtt tcttgcagtc tttctcgggg tcttcaggtt ctacttaccc
901ctgttcttgc aaacatcctt gaagcagatc aggaaaagtg ttggggtttt gaccagtttt
961ttgcagaaac tagtgatata cttcaccgaa tggtaattca tgttttttcg ctacaacaaa
1021tgacagctca taagatttat atacatagct ataatactgc tactatattt catgaactgg
1081tatataaaca aaccaaaatt atttcttcaa atcaagaact tatctacgaa gggcgacgct
1141tagtcttaga acctggaagg ctggcacaac atttccctaa aactactgag gaaaacccta
1201tatttgtagt aagccgggaa cctctggata ccataggatt aatatatgaa aaaatttccc
1261tccctaaagt acatccacgt tatgatttag acggggatgc tagcatggct aaggcaataa
1321caggggttgt gtgttatgcc tgcagaattg ccagtacctt actgctttat caggaattaa
1381tgcgaaaggg gatacgatgg ctgattgaat taattaaaga tgattacaat gaaactgttc
1441acaaaaagac agaagttgtg atcacattgg atttctgtat cagaaacatt gaaaaaactg
1501tgaaagtata tgaaaagttg atgaagatca acctggaagc ggcagagtta ggtgaaattt
1561cagacataca caccaaattg ttgagacttt ccagttctca gggaacaata gaaaccagtc
1621ttcaggatat cgacagcaga ttatctccag gtggatcact ggcagacgca tgggcacatc
1681aagaaggcac tcatccGAAA GACAGAAATG TAGAAAAACT ACAAGTCCTG TTAAATTGCA
1741TGACAGAGAT TTACTATCAG TTCAAAAAAG ACCAAGCAGA ACGTAGATTA GCTTATAATG
1801AAGAACAAAT CCACAAATTT GATAAGCAAA AACTGTATTA CCATGCCACA AAAGCTATGA
1861CGCACTTTAC AGATGAATGT GTTAAAAAGT ATGAGGCATT TTTGAATAAG TCAGAAGAAT
1921GGATAAGAAA GATGCTTCAT CTTAGGAAAC AGTTATTATC GCTGACTAAT CAGTGTTTTG
1981ATATTGAAGA AGAAGTATCA AAATATCAAG AATATACTAA TGAGTTACAA GAAACTCTGC
2041CTCAGAAAAT GTTTACAGCT TCCAGTGGAA TCAAACATAC CATGACCCCA ATTTATCCAA
2101GTTCTAACAC ATTAGTAGAA ATGACTCTTG GTATGAAGAA ATTAAAGGAA GAGATGGAAG
2161GGGTGGTTAA AGAACTTGCT GAAAATAACC ACATTTTAGA AAGGTTTGGC TCTTTAACCA
2221TGGATGGTGG CCTTCGCAAC GTTGACTGTC TTTACCCAAC TTTCTTGTAC AAAGTGGTTG
2281ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2341GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG
2401GCTGTCTGTA GTGTTCATCT CGACGCGTTA AGTCgacaat caacctctgg attacaaaat
2461ttgtgaaaga tt

Download FASTA (ORF) (Full)