Construct: ORF TRCN0000475438
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000464.1_s317c1
- Derived from:
- ccsbBroadEn_07546
- DNA Barcode:
- CTTTAGCTGAGGCGGCCAGTGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPP3 (10072)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475438
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10072 | DPP3 | dipeptidyl peptidase 3 | NM_005700.5 | 99.8% | 99.8% | 227G>A;993C>T;1938A>G |
| 2 | human | 10072 | DPP3 | dipeptidyl peptidase 3 | NM_130443.4 | 99.8% | 99.8% | 227G>A;993C>T;1938A>G |
| 3 | human | 10072 | DPP3 | dipeptidyl peptidase 3 | NM_001256670.2 | 95.7% | 95.7% | (many diffs) |
| 4 | mouse | 75221 | Dpp3 | dipeptidylpeptidase 3 | NM_133803.2 | 87.5% | 92.6% | (many diffs) |
| 5 | mouse | 75221 | Dpp3 | dipeptidylpeptidase 3 | XM_006531854.3 | 87.5% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2277
- ORF length:
- 2211
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacacccag tacatcctgc ccaatgacat cggcgtgtct agcctggact 121 gccgtgaggc cttccgcctg ctgtcaccca cagagcgcct ctatgcctac cacctgtccc 181 gtgccgcctg gtacggaggc ctggctgtgc tgcttcagac ctcccctgag gccccctaca 241 tctatgctct gctcagccgc ctcttccgcg cccaggaccc cgaccagctg caccaacatg 301 ccctggctga aggccttacc gaggaggagt atcaggcgtt cctggtctat gccgcgggtg 361 tttactccaa catgggcaac tacaagtcct ttggtgacac caagtttgtt cccaacttgc 421 ccaaggaaaa gctggaacgg gtgatcctag ggagtgaggc tgctcagcag cacccagaag 481 aagtcagggg cctctggcag acctgcgggg agcttatgtt ctctctggag ccaaggcttc 541 gacacctcgg actggggaag gagggaatca ccacctattt ctctgggaat tgtaccatgg 601 aagatgccaa attggcccag gactttctgg actcacagaa cctcagtgcc tacaacaccc 661 ggctcttcaa agaggtcgat ggagaaggga agccctacta cgaggtgcgg ctggcttctg 721 tgcttggctc agagccttcc ctggactctg aggtgacttc caagctgaag agctatgaat 781 tccggggaag ccctttccag gtgacccggg gggactacgc gcccatcctc cagaaggtgg 841 tggagcagct ggagaaagcc aaggcctatg cagccaacag ccaccagggg cagatgctgg 901 cccagtatat agagagcttc acccagggct ccatcgaggc ccacaagagg ggctcccgct 961 tctggatcca ggacaaaggc cccatcgtgg agagttacat cgggttcatc gagagctacc 1021 gcgacccctt tggttcccga ggagaatttg aaggttttgt agctgtggtg aacaaggcca 1081 tgagtgccaa gtttgagcgg ctggtggcga gcgcagagca gctgctgaag gagctgccct 1141 ggcccccaac ctttgagaag gacaagttcc tcacccctga cttcacctcc ctggatgttc 1201 tcaccttcgc tggctccggc atccctgccg gcatcaacat ccccaactac gatgatctga 1261 ggcagacgga aggctttaag aacgtgtcgc tggggaatgt gctggctgtg gcctacgcca 1321 cgcagcggga gaagcttacc tttctggagg aggatgacaa ggacctgtac atcctctgga 1381 aggggccctc cttcgatgtg caggtgggcc tgcacgagct gctgggccat ggcagtggca 1441 agctcttcgt acaggacgaa aaaggagcat tcaactttga ccaggaaaca gtgatcaacc 1501 cagagacggg cgagcagatt cagagctggt atcggagcgg ggagacctgg gatagcaagt 1561 tcagcaccat cgcctccagc tacgaagagt gccgggctga gagcgtgggt ctctacctct 1621 gtctccaccc gcaagtgctg gagatctttg gctttgaggg ggctgatgcg gaggacgtga 1681 tctacgtgaa ctggctcaac atggttcggg ccgggctgct cgctctggag ttctacacac 1741 ctgaggcctt caactggcga caggcccata tgcaggcccg gtttgtgatc cTGAGAGTCT 1801 TGCTGGAGGC TGGCGAGGGA CTCGTTACCA TCACTCCCAC CACAGGCTCC GATGGGCGCC 1861 CAGATGCCCG GGTCCGCCTC GACCGCAGCA AGATCCGGTC TGTGGGCAAG CCTGCTCTAG 1921 AGCGCTTCCT GCGGAGACTT CAGGTGCTGA AGTCCACAGG GGATGTGGCC GGAGGGCGGG 1981 CCCTGTACGA GGGGTATGCA ACGGTCACTG ATGCGCCCCC CGAGTGCTTC CTCACCCTCA 2041 GGGACACGGT GCTGCTGCGT AAGGAATCTC GGAAGCTCAT TGTTCAGCCC AACACTCGCC 2101 TTGAAGGCTC AGACGTGCAG CTTCTGGAAT ACGAGGCGTC AGCTGCTGGC CTCATCCGAT 2161 CCTTCTCTGA GCGTTTCCCA GAGGATGGAC CCGAGTTGGA GGAGATCCTC ACACAGCTGG 2221 CCACAGCCGA TGCCCGATTC TGGAAGGGCC CCAGTGAGGC CCCATCTGGC CAAGCTTGCC 2281 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2341 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2401 ATATATCTTG TGGAAAGGAC GACTTTAGCT GAGGCGGCCA GTGGTTACGC GTTAAGTCga 2461 caatcaacct ctggattaca aaatttgtga aagatt