Construct: ORF TRCN0000475441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018292.1_s317c1
- Derived from:
- ccsbBroadEn_04022
- DNA Barcode:
- TCGATCTGTCGCCTACCTTCGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLX1B (79008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 548593 | SLX1A | SLX1 homolog A, structure-s... | NM_001015000.2 | 100% | 100% | |
| 2 | human | 79008 | SLX1B | SLX1 homolog B, structure-s... | NM_178044.2 | 100% | 100% | |
| 3 | human | 548593 | SLX1A | SLX1 homolog A, structure-s... | NM_001014999.2 | 58.5% | 58.5% | 240_581del |
| 4 | human | 79008 | SLX1B | SLX1 homolog B, structure-s... | NM_024044.4 | 58.5% | 58.5% | 240_581del |
| 5 | human | 100526831 | SLX1B-SULT1A4 | SLX1B-SULT1A4 readthrough (... | NR_037609.1 | 17.9% | (many diffs) | |
| 6 | human | 100526830 | SLX1A-SULT1A3 | SLX1A-SULT1A3 readthrough (... | NR_037608.1 | 17.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 552
- ORF length:
- 483
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtcccgcg ggggtcgcgg cgaggccagg gcgctttttc ggcgtctacc 121 tgctctactg cctgaacccc cggtaccggg gccgcgtcta cgtggggttc actgtcaaca 181 ctgctcgtcg ggtccagcag cacaatgggg gccgcaaaaa aggcggggcc tggcggaCCA 241 GCGGGCGAGG GCCCTGGGAG ATGGTGCTCG TCGTGCACGG CTTCCCGTCC TCCGTGGCCG 301 CCCTTCGGGA TGAAGAGGGG CCCTTGTGTT GCCCCCACCC TGGCTGCCTG CTAAGGGCCC 361 ATGTGATCTG CCTGGCAGAG GAGTTTCTTC AGGAAGAACC AGGGCAGCTT CTGCCCCTAG 421 AGGGCCAATG CCCTTGCTGT GAGAAGTCAC TGCTTTGGGG AGACCTGATC TGGCTGTGCC 481 AGATGGACAC TGAGAAAGAA GTAGAAGACT CAGAATTAGA AGAGGCACAC TGGACAGACC 541 TGCTGGAGAC CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCG ATCTGTCGCC TACCTTCGTT 721 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t