Construct: ORF TRCN0000475449
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006593.1_s317c1
- Derived from:
- ccsbBroadEn_02645
- DNA Barcode:
- CAGCAGCTTTATACTTGAGCCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABCB8 (11194)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475449
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11194 | ABCB8 | ATP binding cassette subfam... | NM_007188.5 | 100% | 100% | |
| 2 | human | 11194 | ABCB8 | ATP binding cassette subfam... | NM_001282291.2 | 97.6% | 97.6% | 96_146del |
| 3 | human | 11194 | ABCB8 | ATP binding cassette subfam... | NM_001282292.2 | 96.5% | 96.5% | 1940_1941ins75 |
| 4 | human | 11194 | ABCB8 | ATP binding cassette subfam... | NM_001282293.1 | 83.5% | 83.5% | 1_49del;143_144ins313 |
| 5 | mouse | 74610 | Abcb8 | ATP-binding cassette, sub-f... | XM_011249790.2 | 52.1% | 54.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2223
- ORF length:
- 2154
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggtgcat ttatttcggg tcgggattcg gggtggccca ttcccaggca 121 ggctgctacc gcccctccgc ttccagacat tctcagctgt caggtactct gatggctacc 181 gcagctcctc cctcctccgg gccgtggccc acctgcggtc ccagctctgg gcccacctcc 241 ctcgagcccc cctagctccc agatggagcc cctctgcctg gtgctgggtt gggggagccc 301 tgctaggccc catggtactg agtaagcatc cccacctctg ccttgtggcc ctgtgtgagg 361 cagaagaggc ccctcctgcc agctccacac cccatgtcgt ggggtctcgc tttaactgga 421 agctcttctg gcagtttctg cacccccacc tgctggtcct gggggtagcc gtcgtgctgg 481 ccttgggtgc ggcactcgtg aatgtacaga tccccctgct cctgggccag ctggtagagg 541 tcgtggccaa gtacacaagg gaccacgtag ggagtttcat gactgagtcc cagaatctca 601 gcacccacct gcttatcctc tatggtgtcc agggactgct gaccttcggg tacctggtgc 661 tgctgtccca cgttggcgag cgcatggctg tggacatgcg gagggccctc ttcagctccc 721 tgctccgaca agacatcacc ttctttgacg ccaataagac agggcagctg gtgagccgct 781 tgacaactga cgtgcaggag tttaagtcat ccttcaagct tgtcatctcc caggggctgc 841 gaagctgcac ccaggtggca ggctgcctgg tgtccctgtc catgctgtcg acacgcctca 901 cgctgctgct gatggtggcc acaccagccc tgatgggagt gggcaccctg atgggctcag 961 gcctccgaaa attgtctcgc cagtgtcagg agcagatcgc cagggcaatg ggcgtagcag 1021 acgaggccct gggcaatgtg cggactgtgc gtgccttcgc catggagcaa cgggaagagg 1081 agcgctatgg ggcagagctg gaagcctgcc gctgccgggc agaggagctg ggccgcggca 1141 tcgccttgtt ccaagggctt tccaacatcg ccttcaactg catggtcttg ggtaccctat 1201 ttattggggg ctcccttgtg gccggacagc agctgacagg gggagacctc atgtccttcc 1261 tggtggcctc ccagacagtg caaaggtcca tggccaacct ctctgtcctg tttgggcagg 1321 tggtccgggg gctgagtgca ggtgcccggg tctttgagta catggccctg aacccctgca 1381 tcccactgtc tgggggctgc tgcgtcccca aagagcagct gcgtggctcc gttacatttc 1441 agaacgtctg cttcagctac ccctgccgcc ccggcttcga ggtgctgaaa gacttcaccc 1501 tgacgctgcc ccctggcaag atcgtggccc tcgtgggcca gtctggcgga ggaaagacca 1561 ccgtggcttc cctgctggag cgcttctacg accccacggc aggcgtggtg atgctggatg 1621 ggcgggacct gcgcaccctt gacccctcct ggctccgggg ccaggttgtc ggcttcatca 1681 gccaggagcc cgtcctgttt gggacgacca tcatggaaaa catccgcttt gggaagctgg 1741 aagcttccga tgaagaggtg tacacagccg cccgggaagc gaatgctcac gagttcatca 1801 ccagcTTCCC CGAGGGCTAC AACACGGTCG TCGGTGAACG GGGCACTACC CTGTCTGGGG 1861 GCCAGAAGCA GCGCCTGGCC ATCGCCCGAG CCCTTATCAA GCAGCCCACG GTGCTGATAC 1921 TGGATGAAGC TACCAGCGCG CTGGATGCAG AGTCCGAGCG GGTTGTACAG GAGGCCCTGG 1981 ACCGGGCCAG TGCAGGCCGC ACGGTGCTGG TAATTGCCCA CCGGCTCAGC ACTGTCCGTG 2041 GGGCCCACTG CATTGTCGTC ATGGCCGATG GCCGTGTCTG GGAGGCTGGG ACACATGAAG 2101 AGCTCCTGAA GAAAGGCGGG CTATACGCCG AGCTCATCCG GAGGCAGGCC CTGGATGCCC 2161 CGAGGACAGC GGCCCCACCG CCCAAAAAGC CAGAAGGCCC CAGGAGCCAC CAGCACAAGT 2221 CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2281 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2341 GGCTTTATAT ATCTTGTGGA AAGGACGACA GCAGCTTTAT ACTTGAGCCT TCACGCGTTA 2401 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt