Construct: ORF TRCN0000475453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009829.1_s317c1
- Derived from:
- ccsbBroadEn_10926
- DNA Barcode:
- AGAGTCCTTCGAAGATTGCCAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITGA9 (3680)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475453
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3680 | ITGA9 | integrin subunit alpha 9 | NM_002207.3 | 60.2% | 59.3% | (many diffs) |
| 2 | mouse | 104099 | Itga9 | integrin alpha 9 | NM_133721.2 | 51.8% | 52.9% | (many diffs) |
| 3 | mouse | 104099 | Itga9 | integrin alpha 9 | XM_006511918.3 | 34.1% | 34.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1962
- ORF length:
- 1896
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cggcccggct gcgccgaggg gcgccgggag gctccgcgcg ctgctgctgg 121 cgctggtggt cgcggggatc cccgcgggcg cctacaacct cgacccgcag cgccccgtgc 181 acttccaggg ccccgctgac tcgttcttcg gctacgcagt tctggagcat ttccacgaca 241 acacgcgctg ggtccttgtg ggcgcaccaa aggcagattc caaatacagc ccttcagtga 301 agtctcctgg ggctgtgttt aagtgccgtg ttcacaccaa ccctgaccgg agatgcaccg 361 aactggacat ggctcgaggg aagaatcggg gcacgtcctg cggaaagacc tgccgggaag 421 accgcgatga tgagtggatg ggggtgagcc tggcccgaca gcccaaggct gatggccgtg 481 tgttggcctg tgctcatcgc tggaagaaca tctactatga agccgaccac atcctacccc 541 atggcttctg ctacatcatc ccctccaacc tccaggccaa aggcaggacg ctgatccctt 601 gctatgaaga gtataagaag aagtacggag aggaacacgg ctcctgccag gctgggatag 661 cgggcttctt caccgaggag ctggtggtga tgggtgctcc agggtcattt tattgggctg 721 gaaccatcaa agtgctgaac cttacggaca acacctattt aaaactgaac gacgaagtga 781 tcatgaacag gcggtacacc tacctgggct acgcagtgac cgctggccac ttctctcacc 841 cgtccaccat tgatgtggta ggaggtgccc cacaggacaa aggcatcggc aaggtttata 901 ttttcagagc tgaccgaaga tcaggcacct taattaagat ctttcaagca tcaggtaaaa 961 agatgggctc ttacttcggc tcctccttgt gcgcagttga cctgaatggg gacggcctct 1021 ctgacctgct ggtgggggcc cccatgtttt ctgagatcag ggatgaggga caggtcactg 1081 tctacatcaa cagaggaaat ggagccctcg aggagcagct ggctctgact ggggatggtg 1141 cctacaatgc gcactttgga gagagcattg ccagcctgga cgatctggac aatgatgggt 1201 tcccagatgt ggccattggt gcacccaagg aggatgactt cgcaggggcg gtctatatct 1261 atcatggtga tgccggtggg atagtccctc agtactcaat gaaactgtct gggcagaaga 1321 taaatccagt gctccggatg tttggtcagt ccatatcggg aggcattgat atggatggaa 1381 atggctatcc tgatgtcact gttggagcct tcatgtccga cagcgtggtt cttctcagag 1441 caaggcctgt cattacggtg gatgtctcca tcttcctccc gggctccatc aacatcacag 1501 cgcctcagtg tcacgacgga cagcagcctg tgaactgcct gaacgtcacc acctgcttca 1561 gcttccatgg caaacacgtt ccagaagaga ttggccTGAA TTATGTTCTG ATGGCTGACG 1621 TGGCCAAAAA GGAGAAGGGC CAGATGCCCA GGGTCTACTT TGTGCTGCTG GGAGAGACCA 1681 TGGGTCAGGT CACAGAGAAG CTGCAGCTGA CTTACATGGA GGAGACGTGT CGTCACTATG 1741 TGGCCCATGT GAAGCGGAGG GTGCAGGACG TCATCAGCCC GATCGTGTTT GAAGCAGCCT 1801 ACAGCCTCAG TGAGCATGTG ACTGGAGAGG AGGAGAGGGA ACTGCCGCCT CTGACACCAG 1861 TTCTCCGCTG GAAAAAGGGA CAAAAGATTG CCCAAAAGAA TCAGGTCAGA ACCTTAAAGC 1921 TCATACTCAG CACCCAAGGT GGCAGCTGCA CAGAGCAGAA ATACCCAACT TTCTTGTACA 1981 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2041 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2101 AGGACGAAGA GTCCTTCGAA GATTGCCAAG CACGCGTTAA GTCgacaatc aacctctgga 2161 ttacaaaatt tgtgaaagat t