Construct: ORF TRCN0000475483
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018098.1_s317c1
- Derived from:
- ccsbBroadEn_11574
- DNA Barcode:
- CCATGCAACCCGCGGTTATATAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP3M2 (10947)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475483
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10947 | AP3M2 | adaptor related protein com... | NM_001134296.1 | 64.1% | 63.7% | (many diffs) |
| 2 | human | 10947 | AP3M2 | adaptor related protein com... | NM_006803.4 | 64.1% | 63.7% | (many diffs) |
| 3 | human | 10947 | AP3M2 | adaptor related protein com... | XM_017012977.2 | 64.1% | 63.7% | (many diffs) |
| 4 | human | 10947 | AP3M2 | adaptor related protein com... | XR_001745459.2 | 22.2% | (many diffs) | |
| 5 | mouse | 64933 | Ap3m2 | adaptor-related protein com... | NM_001122820.1 | 56.8% | 63% | (many diffs) |
| 6 | mouse | 64933 | Ap3m2 | adaptor-related protein com... | NM_029505.3 | 56.8% | 63% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 885
- ORF length:
- 819
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ccatagtctt ttcttgatca actcctctgg agacattttc ctggagaaac 121 attggaaaag tgtggtcagc cgttctgttt gtgattactt ttttgaggcg caagagagag 181 ctactgaggc agaaaatgtg cctccggtta tccctacccc tcaccactat ctcttaagtg 241 tttaccgcca caagatcttt tttgtggccg tgatccagac ggaggtcccc cctctgtttg 301 tcattgagtt tcttcaccga gtggtggaca catttcagga ttattttgga gtctgttcag 361 agccagtgat caaagacaat gtagttgtgg tttatgaggt attggaagag atgcttgaca 421 atggttttcc attggctacc gagtcgaaca ttcttaaaga actcataaag cctcctacca 481 tccttcgaac ggttgtcaac accatcacag gaagcacgaa tgtgggtgac cagcttccca 541 ctgGGCAGCT GTCAGTGGTG CCTTGGCGAC GGACTGGGGT GAAATATACC AACAATGAGG 601 CCTATTTTGA TGTGATTGAA GAGATTGATG CAATTATTGA TAAATCAGGC TCCACAATTA 661 CTGCTGAGAT CCAGGGGGTG ATTGATGCCT GTGTCAAGCT GACTGGCATG CCAGACCTTA 721 CACTTTCCTT CATGAACCCT AGGTTGTTGG ATGATGTCAG CTTCCATCCT TGTGTTCGTT 781 TCAAACGCTG GGAATCTGAG CGCATCCTCT CCTTCATCCC TCCTGATGGA AACTTCCGCC 841 TGCTGTCTTA CCATGTCAGT GCACAGAAAT GCTGTCTTGG GATGTACCCA ACTTTCTTGT 901 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 961 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1021 GAAAGGACGA CCATGCAACC CGCGGTTATA TAATACGCGT TAAGTCgaca atcaacctct 1081 ggattacaaa atttgtgaaa gatt