Construct: ORF TRCN0000475502
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010166.1_s317c1
- Derived from:
- ccsbBroadEn_04436
- DNA Barcode:
- GTGAGATATTAACGAGACCCATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RSPO3 (84870)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475502
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84870 | RSPO3 | R-spondin 3 | NM_032784.5 | 100% | 100% | |
| 2 | human | 84870 | RSPO3 | R-spondin 3 | XM_017011378.1 | 79.6% | 78.6% | (many diffs) |
| 3 | human | 84870 | RSPO3 | R-spondin 3 | XM_017011379.1 | 74.6% | 74.6% | 0_1ins207 |
| 4 | mouse | 72780 | Rspo3 | R-spondin 3 | NM_028351.3 | 89.3% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 882
- ORF length:
- 816
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cttgcgactg atttcttggc tttttatcat tttgaacttt atggaataca 121 tcggcagcca aaacgcctcc cggggaaggc gccagcgaag aatgcatcct aacgttagtc 181 aaggctgcca aggaggctgt gcaacatgct cagattacaa tggatgtttg tcatgtaagc 241 ccagactatt ttttgctctg gaaagaattg gcatgaagca gattggagta tgtctctctt 301 catgtccaag tggatattat ggaactcgat atccagatat aaataagtgt acaaaatgca 361 aagctgactg tgatacctgt ttcaacaaaa atttctgcac aaaatgtaaa agtggatttt 421 acttacacct tggaaagtgc cttgacaatt gcccagaagg gttggaagcc aacaaccata 481 ctatggagtG TGTCAGTATT GTGCACTGTG AGGTCAGTGA ATGGAATCCT TGGAGTCCAT 541 GCACGAAGAA GGGAAAAACA TGTGGCTTCA AAAGAGGGAC TGAAACACGG GTCCGAGAAA 601 TAATACAGCA TCCTTCAGCA AAGGGTAACC TGTGTCCCCC AACAAATGAG ACAAGAAAGT 661 GTACAGTGCA AAGGAAGAAG TGTCAGAAGG GAGAACGAGG AAAAAAAGGA AGGGAGAGGA 721 AAAGAAAAAA ACCTAATAAA GGAGAAAGTA AAGAAGCAAT ACCTGACAGC AAAAGTCTGG 781 AATCCAGCAA AGAAATCCCA GAGCAACGAG AAAACAAACA GCAGCAGAAG AAGCGAAAAG 841 TCCAAGATAA ACAGAAATCG GTATCAGTCA GCACTGTACA CTACCCAACT TTCTTGTACA 901 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 961 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1021 AGGACGAGTG AGATATTAAC GAGACCCATC AACGCGTTAA GTCgacaatc aacctctgga 1081 ttacaaaatt tgtgaaagat t