Construct: ORF TRCN0000475515
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003626.1_s317c1
- Derived from:
- ccsbBroadEn_13281
- DNA Barcode:
- TTGAAGCACTGCCTCCCTGGTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC12 (151903)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475515
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151903 | CCDC12 | coiled-coil domain containi... | NM_001277074.1 | 99.7% | 100% | 402C>T |
| 2 | human | 151903 | CCDC12 | coiled-coil domain containi... | NM_144716.5 | 92.5% | 92.7% | 1_39del;441C>T |
| 3 | human | 151903 | CCDC12 | coiled-coil domain containi... | XM_024453364.1 | 92.3% | 92.7% | 1_39del;48A>G;441C>T |
| 4 | human | 151903 | CCDC12 | coiled-coil domain containi... | XM_011533396.2 | 84.6% | 82.5% | (many diffs) |
| 5 | human | 151903 | CCDC12 | coiled-coil domain containi... | NR_102269.1 | 49.7% | (many diffs) | |
| 6 | human | 151903 | CCDC12 | coiled-coil domain containi... | XR_002959494.1 | 6.4% | (many diffs) | |
| 7 | human | 151903 | CCDC12 | coiled-coil domain containi... | XR_002959493.1 | 6.3% | (many diffs) | |
| 8 | mouse | 72654 | Ccdc12 | coiled-coil domain containi... | NM_028312.3 | 87.7% | 90.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 564
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcgactacg gctggtgtgg gccggctaga ggaagaggcg ttgcggcgaa 121 aggaacggct gaaggcccta cgggagaaaa ccgggcgcaa ggacaaggaa gatggggagc 181 caaagaccaa gcatcTCAGA GAAGAGGAGG AAGAAGGCGA GAAGCACAGG GAACTTAGGC 241 TGCGGAACTA TGTCCCGGAG GATGAGGACC TGAAGAAGAG GAGGGTGCCC CAGGCCAAAC 301 CGGTTGCAGT GGAGGAGAAG GTGAAGGAGC AGCTGGAGGC CGCCAAGCCC GAGCCCGTCA 361 TCGAGGAGGT GGACCTGGCC AACCTCGCTC CTCGGAAGCC TGACTGGGAC CTCAAGAGAG 421 ATGTGGCCAA GAAGCTGGAG AAACTAAAAA AGCGGACTCA GAGGGCTATT GCCGAGCTGA 481 TCCGTGAAAG GCTGAAAGGC CAGGAAGACA GCCTAGCCTC TGCAGTGGAT GCTGCCACCG 541 AACAAAAGAC CTGTGACTCC GACTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TGAAGCACTG 721 CCTCCCTGGT CAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att