Construct: ORF TRCN0000475516
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010207.1_s317c1
- Derived from:
- ccsbBroadEn_13156
- DNA Barcode:
- GTACATAGGTGTTTTATTCAGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM182 (130827)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475516
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130827 | TMEM182 | transmembrane protein 182 | NM_001321345.2 | 82.4% | 81.9% | 1_69del;379T>C |
| 2 | human | 130827 | TMEM182 | transmembrane protein 182 | NM_001321346.2 | 74% | 66.6% | (many diffs) |
| 3 | human | 130827 | TMEM182 | transmembrane protein 182 | NM_001321343.2 | 58.9% | 58.6% | 1_228del;538T>C |
| 4 | human | 130827 | TMEM182 | transmembrane protein 182 | NM_001321344.2 | 58.9% | 58.6% | 1_228del;538T>C |
| 5 | human | 130827 | TMEM182 | transmembrane protein 182 | XM_017003375.1 | 53.2% | 43.1% | (many diffs) |
| 6 | human | 130827 | TMEM182 | transmembrane protein 182 | NM_144632.5 | 47.8% | 47.5% | 1_357del;667T>C |
| 7 | human | 130827 | TMEM182 | transmembrane protein 182 | XM_011510632.2 | 22.3% | 17.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 396
- ORF length:
- 330
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cctgggggta gttgctgtag tcatcgcaag ctttttgatc atctgtgcag 121 cccccTTCGC CAGCCATTTT CTCTACAAAG CTGGGGGAGG CTCATATATT GCTGCAGGCA 181 TCCTATTTTC ATTGGTGGTG ATGCTGTATG TCATCTGGGT CCAGGCAGTG GCTGACATGG 241 AAAGCTACCG AAACATGAAA ATGAAGGACT GCCTGGATTT CACCCCTTCT GTTCTGTATG 301 GCTGGTCATT TTTCCTGGCC CCAGCTGGGA TATTTTTTTC TTTGCTAGCT GGATTACTAT 361 TTCTGGTTGT TGGACGGCAT ATTCAGATAC ATCACTACCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 AGTACATAGG TGTTTTATTC AGTGAACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt