Transcript: Human NM_001321345.2

Homo sapiens transmembrane protein 182 (TMEM182), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
TMEM182 (130827)
Length:
3348
CDS:
236..637

Additional Resources:

NCBI RefSeq record:
NM_001321345.2
NBCI Gene record:
TMEM182 (130827)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321345.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000369119 CTATGACTCTGCAGTTATTTA pLKO_005 262 CDS 100% 15.000 21.000 N TMEM182 n/a
2 TRCN0000369063 GGTCGCAAAGGCCTGATAATA pLKO_005 915 3UTR 100% 15.000 21.000 N TMEM182 n/a
3 TRCN0000364505 ATTGCTGCAGGCATCCTATTT pLKO_005 407 CDS 100% 13.200 9.240 N TMEM182 n/a
4 TRCN0000377272 CTTCTGGAGGTGTTGGTTTAA pLKO_005 112 5UTR 100% 13.200 9.240 N TMEM182 n/a
5 TRCN0000376483 TTTGCTAGCTGGATTACTATT pLKO_005 580 CDS 100% 13.200 9.240 N TMEM182 n/a
6 TRCN0000413355 CATTGGTGGTGATGCTGTATG pLKO_005 429 CDS 100% 10.800 7.560 N TMEM182 n/a
7 TRCN0000005751 GTGGAAGAGAATGACTCCAAT pLKO.1 140 5UTR 100% 4.950 3.465 N TMEM182 n/a
8 TRCN0000005752 CTGTTCTGTATGGCTGGTCAT pLKO.1 528 CDS 100% 4.050 2.835 N TMEM182 n/a
9 TRCN0000005749 GCTGGATTACTATTTCTGGTT pLKO.1 587 CDS 100% 2.640 1.848 N TMEM182 n/a
10 TRCN0000005750 GCTGTAGTCATCGCAAGCTTT pLKO.1 323 CDS 100% 4.950 2.970 N TMEM182 n/a
11 TRCN0000005748 CCCTGCTTTATTAAAGACCAT pLKO.1 1575 3UTR 100% 2.640 1.584 N TMEM182 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321345.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13156 pDONR223 100% 82.4% 81.9% None 1_69del;379T>C n/a
2 ccsbBroad304_13156 pLX_304 0% 82.4% 81.9% V5 1_69del;379T>C n/a
3 TRCN0000475516 GTACATAGGTGTTTTATTCAGTGA pLX_317 80.2% 82.4% 81.9% V5 1_69del;379T>C n/a
4 ccsbBroadEn_09530 pDONR223 100% 57.9% 57.6% None 0_1ins288;379T>C n/a
5 ccsbBroad304_09530 pLX_304 0% 57.9% 57.6% V5 0_1ins288;379T>C n/a
6 TRCN0000478198 CGCCTCCACCATATAGCTGTATCT pLX_317 39.5% 57.9% 57.6% V5 0_1ins288;379T>C n/a
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