Construct: ORF TRCN0000475518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002083.1_s317c1
- Derived from:
- ccsbBroadEn_10525
- DNA Barcode:
- ATAGGTTCCCGGATTGCGTTTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TXNDC5 (81567)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475518
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81567 | TXNDC5 | thioredoxin domain containi... | NM_001145549.3 | 89.9% | 90% | 0_1ins108;966C>T |
| 2 | human | 81567 | TXNDC5 | thioredoxin domain containi... | NM_030810.5 | 81.7% | 78.9% | (many diffs) |
| 3 | human | 100526836 | BLOC1S5-TXNDC5 | BLOC1S5-TXNDC5 readthrough ... | NR_037616.1 | 32.1% | 1_375del;1449C>T;1456_3352del | |
| 4 | mouse | 105245 | Txndc5 | thioredoxin domain containi... | NM_001289599.1 | 82.2% | 84.7% | (many diffs) |
| 5 | mouse | 105245 | Txndc5 | thioredoxin domain containi... | NM_001289598.1 | 76.6% | 79.1% | (many diffs) |
| 6 | mouse | 105245 | Txndc5 | thioredoxin domain containi... | NM_145367.4 | 72.9% | 73.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1146
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tcagtctgta gactccaaca gagggaacag gaacgaaaaa aggtgtggac 121 actgccagcg gctgcagccg acttggaatg acctgggaga caaatacaac agcatggaag 181 atgccaaagt ctatgtggct aaagtggact gcacggccca ctccgacgtg tgctccgccc 241 agggggtgcg aggatacccc accttaaagc ttttcaagcc aggccaagaa gctgtgaagt 301 accagggtcc tcgggacttc cagacactgg aaaactggat gctgcagaca ctgaacgagg 361 agccagtgac accagagccg gaagtggaac cgcccagtgc ccccgagctc aagcaagggc 421 tgtatgagct ctcagcaagc aactttgagc tgcacgttgc acaaggcgac cactttatca 481 agttcttcgc tccgtggtgt ggtcactgca aagccctggc tccaacctgg gagcagctgg 541 ctctgggcct tgaacattcc gaaactgtca agattggcaa ggttgattgt acacagcact 601 atgaactctg ctccggaaac caggttcgtg gctatcccac tcttctctgg ttccgagatg 661 ggaaaaaggt ggatcagtac aagggaaagc gggatttgga gtcactgagg gagtacgtgg 721 agtcgcagct gcagcgcaca gagactggag cgacggagac cgtcacgccc tcagaggccc 781 cggtgctggc agctgagccc gaggctgaca agggcactgt gttGGCACTC ACTGAAAATA 841 ACTTCGATGA CACCATTGCA GAAGGAATAA CCTTCATCAA GTTTTATGCT CCATGGTGTG 901 GTCATTGTAA GACTCTGGCT CCTACTTGGG AGGAACTCTC TAAAAAGGAA TTCCCTGGTC 961 TGGCGGGGGT CAAGATCGCC GAAGTAGACT GCACTGCTGA ACGGAATATC TGCAGCAAGT 1021 ATTCGGTACG AGGCTACCCC ACGTTATTGC TTTTCCGAGG AGGGAAGAAA GTCAGTGAGC 1081 ACAGTGGAGG CAGAGACCTT GACTCGTTAC ACCGCTTTGT CCTGAGCCAA GCGAAAGATG 1141 AACTTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1201 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1261 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATAGGTTCC CGGATTGCGT TTCCTACGCG 1321 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt