Construct: ORF TRCN0000475520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000060.1_s317c1
- Derived from:
- BRDN0000396647
- DNA Barcode:
- AATCCCCTTTCCAAGTACGAATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COL5A1 (1289)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475520
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1289 | COL5A1 | collagen type V alpha 1 chain | NM_000093.5 | 32% | 32.1% | (many diffs) |
| 2 | human | 1289 | COL5A1 | collagen type V alpha 1 chain | NM_001278074.1 | 31.6% | 31.5% | (many diffs) |
| 3 | human | 1289 | COL5A1 | collagen type V alpha 1 chain | XM_017014266.2 | 24.8% | 24.6% | (many diffs) |
| 4 | human | 1289 | COL5A1 | collagen type V alpha 1 chain | XR_001746183.1 | 20.9% | (many diffs) | |
| 5 | mouse | 12831 | Col5a1 | collagen, type V, alpha 1 | NM_015734.2 | 28.4% | 31.5% | (many diffs) |
| 6 | mouse | 12831 | Col5a1 | collagen, type V, alpha 1 | XM_006497644.3 | 28% | 30.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1839
- ORF length:
- 1773
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccccccgggt cccccgggcc cccgaggacc ctccggagct ccaggtgctg 121 atggcccaca aggtccccca ggtggaatag gaaaccctgg tgcagtggga gagaagggcg 181 agcctggcga agcaggtgag cctggccttc cgggagaagg cggccccccg ggacccaaag 241 gagaaagggg agagaagggc gagtcaggcc cttcaggtgc tgccggaccc cctggaccca 301 aaggccctcc cggagatgat ggtcccaaag gcagccctgg cccagtgggt tttcctggag 361 atcctggccc ccccggagag cctggccccg cgggtcaaga tggtccccct ggtgacaaag 421 gagatgatgg tgaacccggg cagacaggat cccccggccc tactggtgaa ccaggtccat 481 cggggcctcc aggaaaaagg ggtcccccag gccccgcagg ccccgaaggc agacagggag 541 agaaaggggc caagggagaa gccggcttgg aaggccctcc tgggaagact ggccccatcg 601 gcccccaggg ggcccctggg aagcccggac cggatggcct tcgagggatc cctggccctg 661 tgggagaaca aggtctccca ggatccccag gcccggacgg tccccccggc cccatgggtc 721 ccccaggact tcccggcctc aaaggagatt ctggtcccaa aggtgaaaag ggtcatccag 781 gcctgatcgg gctcatcggt cctcccggtg aacagggtga gaagggcgac cgtggtctcc 841 ctggccccca gggctcctcc ggtcctaagg gagaacaggg tatcactggt ccttctggcc 901 cgattgggcc tcctgggccc cctggcctgc cgggtccgcc tggtccaaaa ggtgctaagg 961 gctcctcggg tccaactggc ccgaagggtg aggcaggcca cccaggaccc ccaggccccc 1021 cgggtccccc gggagaggtc atccagcccc tgccaatcca ggcatccagg acgcggcgga 1081 acatcgacgc cagccagctg ctggacgacg ggaatggcga gaactacgtg gactacgcgg 1141 acggcatgga agagatcttc ggctctctca actctctgaa gctggagatt gagcagatga 1201 aacggcccct gggcacgcag cagaaccccg cccgcacctg caaggacctg cagctctgcc 1261 accccgactt cccagatggt gaatactggg tcgatcctaa ccaaggatgc tccagggatt 1321 ccttcaaggt ttactgcaac ttcacagccg gggggtcgac atgcgtcttc cctgacaaga 1381 agtccgaagg ggccagaatc acttcttggc ccaaagaaaa cccgggctcc tggttcagtg 1441 aattcaagcg tgggaaactg ctctcctatg tggacgccga gggcaaccct gtgggtgtgg 1501 tacagatgac cttcctgcgg ctgctgagcg cctctgccca ccagaacgtc acctaccact 1561 gctaccagtc agtggcctgg caggacgcag ccacgggcag ctacgacaag gccctccgct 1621 tcctgggctc caacgacgag gagatgtcct atgacaacaa cccctacatc cgcgccctgg 1681 tggacggctg tgctaccaag aaaggctacc agaagacggt tctggagatc gacaccccca 1741 aagtggagca ggtgcccatc gtggacatca tgTTCAATGA CTTCGGTGAA GCGTCACAGA 1801 AATTTGGATT TGAAGTGGGG CCGGCTTGCT TCATGGGCTA CCCAACTTTC TTGTACAAAG 1861 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1921 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1981 ACGAAATCCC CTTTCCAAGT ACGAATCAAC GCGTTAAGTC gacaatcaac ctctggatta 2041 caaaatttgt gaaagatt