Construct: ORF TRCN0000475526

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012870.1_s317c1
Derived from:
ccsbBroadEn_08160
DNA Barcode:
GGACACTCCTACTCCAAGGCTAAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SH3KBP1 (30011)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475526
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 30011 SH3KBP1 SH3 domain containing kinas... NM_031892.3 99.9% 99.8% 1709C>T
2 human 30011 SH3KBP1 SH3 domain containing kinas... NM_001353891.2 96.3% 96.2% 520_594del;1784C>T
3 human 30011 SH3KBP1 SH3 domain containing kinas... XM_017029465.2 93.7% 92.3% 0_1ins97;39_40ins26;1586C>T
4 human 30011 SH3KBP1 SH3 domain containing kinas... XM_011545498.3 93.7% 93.6% 520_651del;1841C>T
5 human 30011 SH3KBP1 SH3 domain containing kinas... NM_001353890.2 93.4% 93.3% 1494_1495ins129;1580C>T
6 human 30011 SH3KBP1 SH3 domain containing kinas... NM_001024666.3 92.7% 91.6% (many diffs)
7 human 30011 SH3KBP1 SH3 domain containing kinas... XM_017029461.1 90.3% 88.9% (many diffs)
8 human 30011 SH3KBP1 SH3 domain containing kinas... NM_001353892.2 90% 90% 520_594del;1569_1570ins129;1655C>T
9 human 30011 SH3KBP1 SH3 domain containing kinas... XM_017029460.1 87.9% 86.6% (many diffs)
10 human 30011 SH3KBP1 SH3 domain containing kinas... NM_001353893.1 87.7% 87.6% 0_1ins177;343_417del;1607C>T
11 human 30011 SH3KBP1 SH3 domain containing kinas... XM_011545500.3 87.6% 87.5% 520_651del;1626_1627ins129;1712C>T
12 human 30011 SH3KBP1 SH3 domain containing kinas... XM_017029468.2 87.3% 85.8% (many diffs)
13 human 30011 SH3KBP1 SH3 domain containing kinas... XM_011545499.3 87% 85.9% (many diffs)
14 human 30011 SH3KBP1 SH3 domain containing kinas... XM_017029467.2 84.1% 82.7% (many diffs)
15 human 30011 SH3KBP1 SH3 domain containing kinas... XM_017029464.2 81.8% 80.5% (many diffs)
16 human 30011 SH3KBP1 SH3 domain containing kinas... NM_001353894.1 81.5% 81.4% (many diffs)
17 human 30011 SH3KBP1 SH3 domain containing kinas... NM_001353895.1 79.3% 79.2% (many diffs)
18 human 30011 SH3KBP1 SH3 domain containing kinas... XM_011545502.2 65.6% 62.9% (many diffs)
19 human 30011 SH3KBP1 SH3 domain containing kinas... NM_001184960.2 64% 63.6% (many diffs)
20 human 30011 SH3KBP1 SH3 domain containing kinas... XM_017029469.1 60.7% 60.6% 0_1ins783;926C>T
21 human 30011 SH3KBP1 SH3 domain containing kinas... NM_001353897.2 57.5% 57.1% (many diffs)
22 human 30011 SH3KBP1 SH3 domain containing kinas... XM_011545503.3 53.7% 53.4% (many diffs)
23 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... NM_001135727.2 90.8% 93.9% (many diffs)
24 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_006528926.3 88.3% 91.2% (many diffs)
25 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_006528925.3 87.5% 90.5% (many diffs)
26 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_006528924.3 85.2% 88.1% (many diffs)
27 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_011247849.2 85.2% 88.1% (many diffs)
28 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_017318585.1 84.9% 87.8% (many diffs)
29 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... NM_021389.6 84.4% 86% (many diffs)
30 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_006528929.3 82.3% 85.4% (many diffs)
31 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_017318584.1 81.8% 84.6% (many diffs)
32 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_006528928.3 79.6% 82.3% (many diffs)
33 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_006528927.3 79.2% 80.7% (many diffs)
34 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_017318586.1 67.1% 67.1% (many diffs)
35 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... NM_001135728.2 57.6% 59% (many diffs)
36 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_006528932.2 51.6% 52.9% (many diffs)
37 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... NR_110970.1 36.3% (many diffs)
38 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... XM_011247851.2 33.1% 32.1% (many diffs)
39 mouse 58194 Sh3kbp1 SH3-domain kinase binding p... NR_110969.1 33% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2061
ORF length:
1995
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt ggaggccata gtggagtttg actaccaggc ccagcacgat gatgagctga
121cgatcagcgt gggtgaaatc atcaccaaca tcaggaagga ggatggaggc tggtgggagg
181gacagatcaa cggcaggaga ggtttgttcc ctgacaactt tgtaagagaa ataaagaaag
241agatgaagaa agaccctctc accaacaaag ctccagaaaa gcccctgcac gaagtgccca
301gtggaaactc tttgctgtct tctgaaacga ttttaagaac caataagaga ggcgagcgac
361ggaggcgccg gtgccaggtg gcattcagct acctgcccca gaatgacgat gaacttgagc
421tgaaagttgg cgacatcata gaggtggtag gagaggtaga ggaaggatgg tgggaaggtg
481ttctcaacgg gaagactgga atgtttcctt ccaacttcat caaggagctg tcaggggagt
541cggatgagct tggcatttcc caggatgagc agctatccaa gtcaagttta agggaaacca
601caggctccga gagtgatggg ggtgactcaa gcagcaccaa gtctgaaggt gccaacggga
661cagtggcaac tgcagcaatc cagcccaaga aagttaaggg agtgggcttt ggagacattt
721tcaaagacaa gccaatcaaa ctaagaccaa ggtcaattga agtagaaaat gactttctgc
781cggtagaaaa gactattggg aagaagttac ctgcaactac agcaactcca gactcatcaa
841aaacagaaat ggacagcagg acaaagagca aggattactg caaagtaata tttccatatg
901aggcacagaa tgatgatgaa ttgacaatca aagaaggaga tatagtcact ctcatcaata
961aggactgcat cgacgtaggc tggtgggaag gagagctgaa cggcagacga ggcgtgttcc
1021ccgataactt cgtgaagtta cttccaccgg actttgaaaa ggaagggaat agacccaaga
1081agccaccgcc tccatccgct cctgtcatca aacaaggggc aggcaccact gagagaaaac
1141atgaaattaa aaagatacct cctgaaagac cagaaatgct tccaaacaga acagaagaaa
1201aagaaagacc agagagagag ccaaaactgg atttacagaa gccctccgtt cctgccatac
1261cgccaaaaaa gcctcggcca cctaagacca attctctcag cagacctggc gcactgcccc
1321cgagaaggcc ggagagaccg gtgggtccgc tgacacacac caggggtgac agtccaaaga
1381ttgacttggc cggcagttcg ctatctggca tcctggacaa agatctctcg gaccgcagca
1441atgacattga cttagaaggt tttgactccg tggtatcatc tactgagaaa ctcagtcatc
1501cgaccacaag cagaccaaaa gctacaggga ggcggcctcc gtcccagtcc ctcacatctt
1561catccctttc aagccctgat atcttcgact ccccaagtcc cgaagaggat aaggaggaac
1621acatttcact tgcgcacaga ggagtggacg cgtcaaagaa aacttccaag actgttacca
1681tatcccaagt gtctgacaac aaagcatccc tgccgcccaa gccggggacc atggcagcag
1741gtggcggtgg gccagcccct ctgtcctcag cggtgccctc ccccctgtca tcctctttgg
1801gaacagctgg acacagagcc aactccccgt ctctgttcgg cacggaagga aaaccaaaga
1861tggagcctgc ggccagcagc caggcggccg tggaggagct aaggacacag gtccgcgagc
1921tgaggagcaT CATCGAGACC ATGAAGGACC AGCAGAAACG AGAGATTAAA CAGTTATTGT
1981CTGAGTTGGA TGAAGAGAAG AAAATCCGGC TTCGGTTGCA GATGGAAGTG AACGACATAA
2041AGAAAGCTCT ACAATCAAAA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
2101CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
2161AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGAC ACTCCTACTC
2221CAAGGCTAAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)