Construct: ORF TRCN0000475526
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012870.1_s317c1
- Derived from:
- ccsbBroadEn_08160
- DNA Barcode:
- GGACACTCCTACTCCAAGGCTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3KBP1 (30011)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475526
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | NM_031892.3 | 99.9% | 99.8% | 1709C>T |
| 2 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | NM_001353891.2 | 96.3% | 96.2% | 520_594del;1784C>T |
| 3 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_017029465.2 | 93.7% | 92.3% | 0_1ins97;39_40ins26;1586C>T |
| 4 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_011545498.3 | 93.7% | 93.6% | 520_651del;1841C>T |
| 5 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | NM_001353890.2 | 93.4% | 93.3% | 1494_1495ins129;1580C>T |
| 6 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | NM_001024666.3 | 92.7% | 91.6% | (many diffs) |
| 7 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_017029461.1 | 90.3% | 88.9% | (many diffs) |
| 8 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | NM_001353892.2 | 90% | 90% | 520_594del;1569_1570ins129;1655C>T |
| 9 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_017029460.1 | 87.9% | 86.6% | (many diffs) |
| 10 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | NM_001353893.1 | 87.7% | 87.6% | 0_1ins177;343_417del;1607C>T |
| 11 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_011545500.3 | 87.6% | 87.5% | 520_651del;1626_1627ins129;1712C>T |
| 12 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_017029468.2 | 87.3% | 85.8% | (many diffs) |
| 13 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_011545499.3 | 87% | 85.9% | (many diffs) |
| 14 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_017029467.2 | 84.1% | 82.7% | (many diffs) |
| 15 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_017029464.2 | 81.8% | 80.5% | (many diffs) |
| 16 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | NM_001353894.1 | 81.5% | 81.4% | (many diffs) |
| 17 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | NM_001353895.1 | 79.3% | 79.2% | (many diffs) |
| 18 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_011545502.2 | 65.6% | 62.9% | (many diffs) |
| 19 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | NM_001184960.2 | 64% | 63.6% | (many diffs) |
| 20 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_017029469.1 | 60.7% | 60.6% | 0_1ins783;926C>T |
| 21 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | NM_001353897.2 | 57.5% | 57.1% | (many diffs) |
| 22 | human | 30011 | SH3KBP1 | SH3 domain containing kinas... | XM_011545503.3 | 53.7% | 53.4% | (many diffs) |
| 23 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | NM_001135727.2 | 90.8% | 93.9% | (many diffs) |
| 24 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_006528926.3 | 88.3% | 91.2% | (many diffs) |
| 25 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_006528925.3 | 87.5% | 90.5% | (many diffs) |
| 26 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_006528924.3 | 85.2% | 88.1% | (many diffs) |
| 27 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_011247849.2 | 85.2% | 88.1% | (many diffs) |
| 28 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_017318585.1 | 84.9% | 87.8% | (many diffs) |
| 29 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | NM_021389.6 | 84.4% | 86% | (many diffs) |
| 30 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_006528929.3 | 82.3% | 85.4% | (many diffs) |
| 31 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_017318584.1 | 81.8% | 84.6% | (many diffs) |
| 32 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_006528928.3 | 79.6% | 82.3% | (many diffs) |
| 33 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_006528927.3 | 79.2% | 80.7% | (many diffs) |
| 34 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_017318586.1 | 67.1% | 67.1% | (many diffs) |
| 35 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | NM_001135728.2 | 57.6% | 59% | (many diffs) |
| 36 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_006528932.2 | 51.6% | 52.9% | (many diffs) |
| 37 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | NR_110970.1 | 36.3% | (many diffs) | |
| 38 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | XM_011247851.2 | 33.1% | 32.1% | (many diffs) |
| 39 | mouse | 58194 | Sh3kbp1 | SH3-domain kinase binding p... | NR_110969.1 | 33% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2061
- ORF length:
- 1995
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggaggccata gtggagtttg actaccaggc ccagcacgat gatgagctga 121 cgatcagcgt gggtgaaatc atcaccaaca tcaggaagga ggatggaggc tggtgggagg 181 gacagatcaa cggcaggaga ggtttgttcc ctgacaactt tgtaagagaa ataaagaaag 241 agatgaagaa agaccctctc accaacaaag ctccagaaaa gcccctgcac gaagtgccca 301 gtggaaactc tttgctgtct tctgaaacga ttttaagaac caataagaga ggcgagcgac 361 ggaggcgccg gtgccaggtg gcattcagct acctgcccca gaatgacgat gaacttgagc 421 tgaaagttgg cgacatcata gaggtggtag gagaggtaga ggaaggatgg tgggaaggtg 481 ttctcaacgg gaagactgga atgtttcctt ccaacttcat caaggagctg tcaggggagt 541 cggatgagct tggcatttcc caggatgagc agctatccaa gtcaagttta agggaaacca 601 caggctccga gagtgatggg ggtgactcaa gcagcaccaa gtctgaaggt gccaacggga 661 cagtggcaac tgcagcaatc cagcccaaga aagttaaggg agtgggcttt ggagacattt 721 tcaaagacaa gccaatcaaa ctaagaccaa ggtcaattga agtagaaaat gactttctgc 781 cggtagaaaa gactattggg aagaagttac ctgcaactac agcaactcca gactcatcaa 841 aaacagaaat ggacagcagg acaaagagca aggattactg caaagtaata tttccatatg 901 aggcacagaa tgatgatgaa ttgacaatca aagaaggaga tatagtcact ctcatcaata 961 aggactgcat cgacgtaggc tggtgggaag gagagctgaa cggcagacga ggcgtgttcc 1021 ccgataactt cgtgaagtta cttccaccgg actttgaaaa ggaagggaat agacccaaga 1081 agccaccgcc tccatccgct cctgtcatca aacaaggggc aggcaccact gagagaaaac 1141 atgaaattaa aaagatacct cctgaaagac cagaaatgct tccaaacaga acagaagaaa 1201 aagaaagacc agagagagag ccaaaactgg atttacagaa gccctccgtt cctgccatac 1261 cgccaaaaaa gcctcggcca cctaagacca attctctcag cagacctggc gcactgcccc 1321 cgagaaggcc ggagagaccg gtgggtccgc tgacacacac caggggtgac agtccaaaga 1381 ttgacttggc cggcagttcg ctatctggca tcctggacaa agatctctcg gaccgcagca 1441 atgacattga cttagaaggt tttgactccg tggtatcatc tactgagaaa ctcagtcatc 1501 cgaccacaag cagaccaaaa gctacaggga ggcggcctcc gtcccagtcc ctcacatctt 1561 catccctttc aagccctgat atcttcgact ccccaagtcc cgaagaggat aaggaggaac 1621 acatttcact tgcgcacaga ggagtggacg cgtcaaagaa aacttccaag actgttacca 1681 tatcccaagt gtctgacaac aaagcatccc tgccgcccaa gccggggacc atggcagcag 1741 gtggcggtgg gccagcccct ctgtcctcag cggtgccctc ccccctgtca tcctctttgg 1801 gaacagctgg acacagagcc aactccccgt ctctgttcgg cacggaagga aaaccaaaga 1861 tggagcctgc ggccagcagc caggcggccg tggaggagct aaggacacag gtccgcgagc 1921 tgaggagcaT CATCGAGACC ATGAAGGACC AGCAGAAACG AGAGATTAAA CAGTTATTGT 1981 CTGAGTTGGA TGAAGAGAAG AAAATCCGGC TTCGGTTGCA GATGGAAGTG AACGACATAA 2041 AGAAAGCTCT ACAATCAAAA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2101 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2161 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGAC ACTCCTACTC 2221 CAAGGCTAAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt