Construct: ORF TRCN0000475527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003722.1_s317c1
- Derived from:
- ccsbBroadEn_07477
- DNA Barcode:
- AGTTTAGGTATCGGTATTCACGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SART3 (9733)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9733 | SART3 | spliceosome associated fact... | NM_014706.4 | 99.8% | 100% | 108G>A;1884T>C;2661G>A |
| 2 | human | 9733 | SART3 | spliceosome associated fact... | XM_005269241.5 | 98% | 98.1% | (many diffs) |
| 3 | human | 9733 | SART3 | spliceosome associated fact... | XM_024449284.1 | 68.4% | 68.9% | (many diffs) |
| 4 | mouse | 53890 | Sart3 | squamous cell carcinoma ant... | NM_016926.1 | 83.1% | 87% | (many diffs) |
| 5 | mouse | 53890 | Sart3 | squamous cell carcinoma ant... | XM_006530405.1 | 81.6% | 85.4% | (many diffs) |
| 6 | mouse | 53890 | Sart3 | squamous cell carcinoma ant... | XM_006530406.1 | 70.7% | 74.9% | (many diffs) |
| 7 | mouse | 53890 | Sart3 | squamous cell carcinoma ant... | XM_017320991.1 | 49.6% | 51.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2958
- ORF length:
- 2889
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgactgcg gccgaaacct cggcttcaga acccgaggct gagtccaagg 121 ctgggcccaa ggctgacgga gaggaggatg aggttaaggc ggctaggaca aggagaaagg 181 tgttatcgcg ggctgtggcc gctgcgacat acaagaccat ggggccagcg tgggatcagc 241 aggaggaagg cgtgagcgag agcgatgggg atgagtacgc catggcttcc tccgcggaga 301 gctcccccgg ggagtacgag tgggaatatg acgaagagga ggagaaaaac cagctggaga 361 ttgagagact ggaggagcag ttgtctatca acgtctatga ctacaactgc catgtggact 421 tgatcagact gctcaggctg gaaggggagc ttaccaaggt gaggatggcc cgccagaaga 481 tgagtgaaat ctttcccttg actgaagagc tctggctgga gtggctgcat gacgagatca 541 gcatggccca ggatggcctg gacagagagc acgtgtatga cctctttgag aaagccgtga 601 aggattacat ttgtcctaac atttggctag agtatggcca gtactcagtt ggtgggattg 661 gtcagaaagg tggccttgag aaagttcgct ccgtgtttga aagggctctc tcgtctgttg 721 gtttacatat gaccaaagga ctcgccctct gggaggctta ccgagagttt gaaagtgcga 781 ttgtggaagc tgctcggctt gagaaagtcc acagtctttt ccggcgacag ttggcgatcc 841 cactctatga tatggaggcc acatttgcag agtatgaaga atggtcagaa gacccaatac 901 cagagtcagt aattcagaac tataacaaag cactacagca gctggagaaa tataaaccct 961 atgaagaagc actgttgcag gcagaggcac caaggctggc agaatatcaa gcatatatcg 1021 attttgagat gaaaattggc gatcctgctc gcattcagtt gatctttgag cgcgccctgg 1081 tcgagaactg ccttgtccca gacttatgga tccgttacag tcagtaccta gatcgacaac 1141 tgaaagtaaa ggatttggtt ttatctgtac ataaccgcgc tattagaaac tgcccctgga 1201 cagttgcctt atggagtcgg tacctcttgg ccatggagag acatggagtt gatcatcaag 1261 taatttctgt aaccttcgag aaagctttga atgccggctt catccaggcc actgattatg 1321 tggagatttg gcaggcatac cttgattacc tgaggagaag ggttgatttc aaacaagact 1381 ccagtaaaga gctggaggag ttgagggccg cctttactcg tgccttggag tatctgaagc 1441 aggaggtgga agagcgtttc aatgagagtg gtgatccaag ctgcgtgatt atgcagaact 1501 gggctaggat tgaggctcga ctgtgcaata acatgcagaa agctcgggaa ctctgggata 1561 gcatcatgac cagaggaaat gccaagtacg ccaacatgtg gctagagtat tacaacctgg 1621 aaagagctca tggtgacacc cagcactgcc ggaaggctct gcaccgggcc gtccagtgca 1681 ccagtgacta cccagagcac gtctgcgaag tgttactcac catggagagg acagaaggtt 1741 ctttagaaga ttgggatata gctgttcaga aaactgaaac ccgattagct cgtgtcaatg 1801 agcagagaat gaaggctgca gagaaggaag cagcccttgt gcagcaagaa gaagaaaagg 1861 ctgaacaacg gaaaagagct cgggctgaga agaaagcgtt aaaaaagaag aaaaagatca 1921 gaggcccaga gaagcgcgga gcagatgagg acgatgagaa agagtggggc gatgatgaag 1981 aagagcagcc ttccaaacgc agaagggtcg agaacagcat ccctgcagct ggagaaacac 2041 aaaatgtaga agtagcagca gggcccgctg ggaaatgtgc tgccgtagat gtggagcccc 2101 cttcgaagca gaaggagaag gcagcctccc tgaagaggga catgcccaag gtgctgcacg 2161 acagcagcaa ggacagcatc accgtctttg tcagcaacct gccctacagc atgcaggagc 2221 cggacacgaa gctcaggcca ctcttcgagg cctgtgggga ggtggtccag atccgaccca 2281 tcttcagcaa ccgtggggat ttccgaggtt actgctacgt ggagtttaaa gaagagaaat 2341 cagcccttca ggcactggag atggaccgga aaagtgtaga agggaggcca atgtttgttt 2401 ccccctgtgt ggataagagc aaaaaccccg attttaaggt gttcaggtac agcacttccc 2461 tagagaaaca caagctgttc atctcaggcc tgcctttctc ctgtactaaa gaggaactag 2521 aagaaatctg taaggctcat ggcaccgtga aggacctcag gctggtcacc aaccgggctg 2581 gcaaaccaaa gggcctggcc tacgtggagt atgaaaatga atcccaggcg tcgcaggctg 2641 tgatgaagat ggacggcatg actatcaaag agaacatcat caaagtggca atcagcaacc 2701 ctccTCAGAG GAAAGTTCCA GAGAAGCCAG AGACCAGGAA GGCACCAGGT GGCCCCATGC 2761 TTTTGCCGCA GACATACGGA GCGAGGGGGA AGGGAAGGAC GCAGCTGTCT CTACTGCCTC 2821 GTGCCCTGCA GCGCCCAAGT GCTGCAGCTC CTCAGGCTGA GAACGGCCCT GCCGCGGCTC 2881 CTGCAGTTGC CGCCCCAGCA GCCACCGAGG CACCCAAGAT GTCCAATGCC GATTTTGCCA 2941 AGCTGTTTCT GAGAAAGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3001 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3061 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGTTTAG GTATCGGTAT 3121 TCACGTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt