Transcript: Human XM_005269241.5

PREDICTED: Homo sapiens spliceosome associated factor 3, U4/U6 recycling protein (SART3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SART3 (9733)
Length:
4208
CDS:
20..2965

Additional Resources:

NCBI RefSeq record:
XM_005269241.5
NBCI Gene record:
SART3 (9733)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005269241.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000127501 CGAGAGTTTGAAAGTGCGATT pLKO.1 713 CDS 100% 4.050 5.670 N SART3 n/a
2 TRCN0000344338 CGAGAGTTTGAAAGTGCGATT pLKO_005 713 CDS 100% 4.050 5.670 N SART3 n/a
3 TRCN0000127760 GCAGTTGTCTATCAACGTCTA pLKO.1 328 CDS 100% 4.050 5.670 N SART3 n/a
4 TRCN0000129569 CCGAGAGTTTGAAAGTGCGAT pLKO.1 712 CDS 100% 2.640 3.696 N SART3 n/a
5 TRCN0000149271 GCTCGCATTCAGTTGATCTTT pLKO.1 1052 CDS 100% 5.625 3.938 N SART3 n/a
6 TRCN0000344340 GCTCGCATTCAGTTGATCTTT pLKO_005 1052 CDS 100% 5.625 3.938 N SART3 n/a
7 TRCN0000128080 CGAAACGAGAACACACTGTTT pLKO.1 3247 3UTR 100% 4.950 3.465 N SART3 n/a
8 TRCN0000148744 CGGCATGACTATCAAAGAGAA pLKO.1 2659 CDS 100% 4.950 3.465 N SART3 n/a
9 TRCN0000146268 CGTGGAGTTTAAAGAAGAGAA pLKO.1 2323 CDS 100% 4.950 3.465 N SART3 n/a
10 TRCN0000148806 CGTTTCAATGAGAGTGGTGAT pLKO.1 1460 CDS 100% 4.050 2.835 N SART3 n/a
11 TRCN0000344260 CGTTTCAATGAGAGTGGTGAT pLKO_005 1460 CDS 100% 4.050 2.835 N SART3 n/a
12 TRCN0000147508 GCTGGAGAAATATAAACCCTA pLKO.1 946 CDS 100% 2.640 1.848 N SART3 n/a
13 TRCN0000344339 GCTGGAGAAATATAAACCCTA pLKO_005 946 CDS 100% 2.640 1.848 N SART3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005269241.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07477 pDONR223 100% 98% 98.1% None (many diffs) n/a
2 ccsbBroad304_07477 pLX_304 0% 98% 98.1% V5 (many diffs) n/a
3 TRCN0000475527 AGTTTAGGTATCGGTATTCACGTG pLX_317 9% 98% 98.1% V5 (many diffs) n/a
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